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Creative Biolabs has successfully developed SARS, MERS and other anti-viral antibodies in the past to assist scientific research. Facing the difficult COVID-19, Creative Biolabs actively invests in antibody development to assist researchers to better understand the characteristics of 2019-nCoV and drug development. Currently, Creative Biolabs is proud to offer an extensive line of research antibodies to support the study of SARS-CoV-2/COVID-19, several of which were validated using virus-infected cell lysates.

Explore the collections of SARS-CoV-2/COVID-19 antibodies >

Human Apoptosis Antibody Array (Membrane, 35 Targets) (Y0233) (AbAr-0233-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Apoptosis Antibody Array detects 35 human apoptosis-related proteins simultaneously.
Specifications
Target Array
Apoptosis Array
Target
Bad, TRAIL R1/DR4, PON2, Bax, TRAIL R2/DR5, p21/CIP1/CDNK1A, Bcl-2, FADD, p27/Kip1, Bcl-x, Fas/TNFSF6, Phospho-p53 (S15), Pro-Caspase-3, HIF-1 alpha, Phospho-p53 (S46), Cleaved Caspase-3, HO-1/HMOX1/HSP32, Phospho-p53 (S392), Catalase, HO-2/HMOX2, Phospho-Rad17 (S635), cIAP-1, HSP27, Pro-Caspase-3, cIAP-2, HSP60, SMAC/Diablo, Claspin, HSP70, Survivin, Clusterin, HTRA2/Omi, TNF RI/TNFRSF1A, Cytochrome c, Livin, XIAP
Species Reactivity
Human
Sample Type
Cell Lysates
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. The average adult human loses between 50 and 70 billion cells each day due to apoptosis. For an average human child between the ages of 8 to 14 year old approximately 20 to 30 billion cells die per day.
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