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Human Apoptosis Antibody Array (Glass Slide, 43 Targets) (Y0014) (AbAr-0014-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Apoptosis Antibody Array detects 43 Human Apoptotic Factors. It is suitable for all liquid sample types but intended for use with cell and tissue lysates.
Specifications
Target Array
Apoptosis Array
Target
bad, BIM, cIAP-2, HSP27, IGF-2, IGFBP-5, p27, TNF RII (TNFRSF1B), TRAIL R3 (TNFRSF10C), bax, Caspase-3, Cytochrome C, HSP60, IGFBP-1, IGFBP-6, p53, TNF alpha, TRAIL R4 (TNFRSF10D), bcl-2, Caspase-8, DR6 (TNFRSF21), HSP70, IGFBP-2, IGF-1 R, SMAC, TNF beta (TNFSF1B), XIAP, bcl-w, CD40 (TNFRSF5), Fas (TNFRSF6/Apo-1), HTRA2, IGFBP-3, livin, Survivin (BIRC5), TRAIL R1 (TNFRSF10A/DR4), BID, CD40 Ligand (TNFSF5), Fas Ligand (TNFSF6), IGF-1, IGFBP-4, p21, TNF RI (TNFRSF1A), TRAIL R2 (TNFRSF10B/DR5)
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Applications
Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. The average adult human loses between 50 and 70 billion cells each day due to apoptosis. For an average human child between the ages of 8 to 14 year old approximately 20 to 30 billion cells die per day.
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For Research Use Only. Not For Clinical Use.