Canine Recombinant ANGPTL7 protein, His Tag (V2LY-0526-LY1203)

Name: Canine Recombinant ANGPTL7 protein, His Tag
SKU: V2LY-0526-LY1203
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Canine

Tag

His Tag

Protein Construction

This product is Canine Recombinant ANGPTL7 protein, His Tag consist of Amino Acid: 1-344 and predicts a molecular mass of 38.2 kDa.

Molecule Mass

38.2 kDa

Sequence

Amino Acid: 1-344

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Angiopoietin Like 7

Function

Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure (PubMed:21199193). Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency (PubMed:25622036).

Biological Process

Angiogenesis Source: GO_Central
Negative regulation of vasculature development involved in avascular cornea development in camera-type eye Source: UniProtKB
Regulation of extracellular matrix organization Source: UniProtKB
Response to oxidative stress Source: ProtInc

Cellular Location

Secreted

Xia, X., Ren, Z., Yan, L., Zheng, X., Yang, H., Kang, M., ... & Yang, J. (2020). Cord Blood Levels of Angiopoietin-Like 7 (ANGPTL7) in Preterm Infants. BioMed Research International, 2020.

Li, J., Liang, T., Wang, Y., Gan, Y., Kou, Z., Zhang, Y., ... & Xie, D. (2020). Angiopoietin-like protein 7 mediates TNF-α-induced adhesion and oxidative stress in human umbilical vein epithelial cell. General physiology and biophysics, 39(3), 285-292.

Zhang, C., He, X., Zhao, J., Cao, Y., Liu, J., Liang, W., ... & Liu, C. (2020). Angiopoietin-like protein 7 and short-term mortality in acute heart failure. Cardiorenal medicine, 10(2), 116-124.

Zhao, Y., Liu, K., Yin, D., & Lin, Z. (2019). Angiopoietin-like 7 contributes to angiotensin II-induced proliferation, inflammation and apoptosis in vascular smooth muscle cells. Pharmacology, 104(5-6), 226-234.

Lu, X., Lu, J., Zhang, L., & Xu, Y. (2019). Effect of ANGPTL7 on Proliferation and Differentiation of MC3T3-E1 Cells. Medical science monitor: international medical journal of experimental and clinical research, 25, 9524.

Abu-Farha, M., Cherian, P., Al-Khairi, I., Madhu, D., Tiss, A., Warsam, S., ... & Abubaker, J. (2017). Plasma and adipose tissue level of angiopoietin-like 7 (ANGPTL7) are increased in obesity and reduced after physical exercise. PloS one, 12(3), e0173024.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant ANGPTL7 protein, hFc Tag (CAT#: V2LY-0526-LY1202)

Rhesus Recombinant ANGPTL7 protein, His Tag (CAT#: V2LY-0526-LY9649)

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Canine Recombinant ANGPTL7 protein, hFc Tag (CAT#: V2LY-0526-LY1202)

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Rhesus Recombinant ANGPTL7 protein, His Tag (CAT#: V2LY-0526-LY9649)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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