Canine Recombinant CD274 protein, ECD, hFc Tag (V2LY-0526-LY1307)

Name: Canine Recombinant CD274 protein, ECD, hFc Tag
SKU: V2LY-0526-LY1307
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Canine

Tag

hFc Tag

Protein Construction

This product is Canine Recombinant CD274 protein, ECD, hFc Tag consist of Amino Acid: 1-236 and predicts a molecular mass of 51.5 kDa.

Molecule Mass

51.5 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-236

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD274 Molecule

Alternative Names

CD274 Molecule; CD274 Antigen; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; PDCD1LG1; PDCD1L1;

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410).
As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410).
Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410).
The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity).
The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Cell surface receptor signaling pathway Source: UniProtKB
Cellular response to lipopolysaccharide Source: GO_Central
Immune response Source: UniProtKB
Negative regulation of activated T cell proliferation Source: UniProtKB
Negative regulation of CD4-positive, alpha-beta T cell proliferation Source: UniProtKB
Negative regulation of CD8-positive, alpha-beta T cell activation Source: UniProtKB
Negative regulation of interferon-gamma production Source: UniProtKB
Negative regulation of interleukin-10 production Source: UniProtKB
Negative regulation of T cell proliferation Source: GO_Central
Negative regulation of tumor necrosis factor superfamily cytokine production Source: UniProtKB
Positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: UniProtKB
Positive regulation of cell migration Source: Ensembl
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of tolerance induction to tumor cell Source: UniProtKB
Response to cytokine Source: AgBase
Signal transduction Source: UniProtKB
T cell costimulation Source: UniProtKB
Toxin transport Source: Ensembl

Cellular Location

Cell membrane; Early endosome membrane; Recycling endosome membrane. Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation.
Isoform 1: Cell membrane
Isoform 2: Endomembrane system

Involvement in disease

Truncation of the 3'-untranslated (3'-UTR) region of CD274 transcripts leads to elevated expression of CD274 in multiple cancers including T-cell leukemia, diffuse large B-cell lymphoma and stomach adenocarcinoma (PubMed:27281199). Disruption of 3'-UTR region is caused by structural variants that stabilize CD274 transcripts, leading to overexpression (PubMed:27281199). Increased expression in tumors promotes immune evasion and tumor cell growth by allowing malignant cells to escape destruction by the immune system (PubMed:27281199).

Topology

Extracellular: 19-238
Helical: 239-259
Cytoplasmic: 260-290

PTM

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.

Verocq, C., Decaestecker, C., Rocq, L., De Clercq, S., Verrellen, A., Mekinda, Z., ... & D'haene, N. (2020). The daily practice reality of PD‑L1 (CD274) evaluation in non‑small cell lung cancer: A retrospective study. Oncology letters, 19(5), 3400-3410.

Riemann, D., Schütte, W., Turzer, S., Seliger, B., & Möller, M. (2020). High PD-L1/CD274 Expression of Monocytes and Blood Dendritic Cells Is a Risk Factor in Lung Cancer Patients Undergoing Treatment with PD1 Inhibitor Therapy. Cancers, 12(10), 2966.

Kim, J. Y., Lee, K. H., Kang, J., Borcoman, E., Saada-Bouzid, E., Kronbichler, A., ... & Gamerith, G. (2019). Hyperprogressive disease during anti-PD-1 (PDCD1)/PD-L1 (CD274) therapy: a systematic review and meta-analysis. Cancers, 11(11), 1699.

Fabrizio, F. P., Trombetta, D., Rossi, A., Sparaneo, A., Castellana, S., & Muscarella, L. A. (2018). Gene code CD274/PD-L1: from molecular basis toward cancer immunotherapy. Therapeutic advances in medical oncology, 10, 1758835918815598.

Huang, C. Y., Chiang, S. F., Ke, T. W., Chen, T. W., You, Y. S., Chen, W. T. L., & Chao, K. C. (2018). Clinical significance of programmed death 1 ligand-1 (CD274/PD-L1) and intra-tumoral CD8+ T-cell infiltration in stage II–III colorectal cancer. Scientific reports, 8(1), 1-10.

Clavé, S., Pijuan, L., Casadevall, D., Taus, Á., Gimeno, J., Hernández‐Llodrà, S., ... & Salido, M. (2018). CD 274 (PDL 1) and JAK 2 genomic amplifications in pulmonary squamous‐cell and adenocarcinoma patients. Histopathology, 72(2), 259-269.

Yang, L., Cai, Y., Zhang, D., Sun, J., Xu, C., Zhao, W., ... & Pan, C. (2018). miR-195/miR-497 regulate CD274 expression of immune regulatory ligands in triple-negative breast cancer. Journal of breast cancer, 21(4), 371-381.

Sun, J., Lian, M., Ma, H., Wang, R., Ma, Z., Wang, H., ... & Fang, J. (2018). Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma. Molecular medicine reports, 17(3), 3859-3869.

Lee, S. J., Jun, S. Y., Lee, I. H., Kang, B. W., Park, S. Y., Kim, H. J., ... & Kim, J. G. (2018). CD274, LAG3, and IDO1 expressions in tumor-infiltrating immune cells as prognostic biomarker for patients with MSI-high colon cancer. Journal of cancer research and clinical oncology, 144(6), 1005-1014.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus, Rhesus Recombinant CD274 protein, Fc Tag (CAT#: V2LY-0526-LY27)

Cynomolgus, Rhesus Recombinant CD274 protein, His Tag (CAT#: V2LY-0526-LY28)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY29)

Canine Recombinant CD274 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY1308)

Canine Recombinant CD274 protein, ECD, His Tag (CAT#: V2LY-0526-LY1309)

Canine Recombinant CD274 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY1310)

Canine Recombinant CD274 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY1311)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY1733)

Cynomolgus, Rhesus Recombinant CD274 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1734)

Feline Recombinant CD274 protein, hFc Tag (CAT#: V2LY-0526-LY1791)

Antibody Pairs

CD274 Matched Antibody Pair (214) (CAT#: APMAB-214LY)

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Human Recombinant CD274 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY6089)

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Canine Recombinant CD274 protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY1308)

Canine Recombinant CD274 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY1311)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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