Canine Recombinant NNMT, Active protein, GST Tag (V2LY-0526-LY1299)

Name: Canine Recombinant NNMT, Active protein, GST Tag
SKU: V2LY-0526-LY1299
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Canine

Tag

GST Tag

Protein Construction

This product is Canine Recombinant NNMT, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 54 kDa.

Molecule Mass

54 kDa

Sequence

Amino Acid: Full Length

Species

Canine

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Nicotinamide N-Methyltransferase

Function

Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed:8182091. PubMed:21823666, PubMed:23455543).
Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed:26571212, PubMed:23455543, PubMed:31043742).
In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed:26571212).
Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD+ levels through the salvage pathway (By similarity).
Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity).
Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed:30044909).

Biological Process

Animal organ regenerationIEA:Ensembl
MethylationTAS:Reactome
NAD biosynthesis via nicotinamide riboside salvage pathwayTAS:Reactome
Nicotinamide metabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of gluconeogenesisISS:UniProtKB
Positive regulation of protein deacetylationISS:UniProtKB
Regulation of histone H3-K4 methylationIEA:Ensembl
Regulation of histone methylationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of polyamine biosynthetic processIEA:Ensembl
Response to organonitrogen compoundIEA:Ensembl
Response to xenobiotic stimulusIEA:Ensembl

Cellular Location

Cytoplasm

PTM

Deiminated by PADI1 and PADI2.

Wang, W., Yang, C., Wang, T., & Deng, H. (2022). Complex roles of nicotinamide N-methyltransferase in cancer progression. Cell Death & Disease, 13(3), 267.

Takahashi, R., Kanda, T., Komatsu, M., Itoh, T., Minakuchi, H., Urai, H., ... & Itoh, H. (2022). The significance of NAD+ metabolites and nicotinamide N-methyltransferase in chronic kidney disease. Scientific reports, 12(1), 6398.

Parsons, R. B., & Facey, P. D. (2021). Nicotinamide N-methyltransferase: an emerging protagonist in cancer macro (r) evolution. Biomolecules, 11(10), 1418.

Roberti, A., Fernández, A. F., & Fraga, M. F. (2021). Nicotinamide N-methyltransferase: At the crossroads between cellular metabolism and epigenetic regulation. Molecular Metabolism, 45, 101165.

Campagna, R., Mateuszuk, Ł., Wojnar-Lason, K., Kaczara, P., Tworzydło, A., Kij, A., ... & Chlopicki, S. (2021). Nicotinamide N-methyltransferase in endothelium protects against oxidant stress-induced endothelial injury. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1868(10), 119082.

Gao, Y., Martin, N. I., & van Haren, M. J. (2021). Nicotinamide N-methyl transferase (NNMT): An emerging therapeutic target. Drug Discovery Today, 26(11), 2699-2706.

Campagna, R., Pozzi, V., Sartini, D., Salvolini, E., Brisigotti, V., Molinelli, E., ... & Emanuelli, M. (2021). Beyond Nicotinamide Metabolism: Potential Role of Nicotinamide N-Methyltransferase as a Biomarker in Skin Cancers. Cancers, 13(19), 4943.

Togni, L., Mascitti, M., Sartini, D., Campagna, R., Pozzi, V., Salvolini, E., ... & Emanuelli, M. (2021). Nicotinamide N-Methyltransferase in head and neck tumors: A comprehensive review. Biomolecules, 11(11), 1594.

Ramsden, D. B., Waring, R. H., Parsons, R. B., Barlow, D. J., & Williams, A. C. (2020). Nicotinamide N-methyltransferase: genomic connection to disease. International Journal of Tryptophan Research, 13, 1178646920919770.

Gao, Y., Van Haren, M. J., Moret, E. E., Rood, J. J., Sartini, D., Salvucci, A., ... & Martin, N. I. (2019). Bisubstrate inhibitors of nicotinamide N-methyltransferase (NNMT) with enhanced activity. Journal of medicinal chemistry, 62(14), 6597-6614.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NNMT, Active protein, GST Tag (CAT#: V2LY-0526-LY5812)

NNMT Matched Antibody Pair (805) (CAT#: APMAB-805LY)

Antibody Pairs

NNMT Matched Antibody Pair (805) (CAT#: APMAB-805LY)

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Human Recombinant NNMT, Active protein, GST Tag (CAT#: V2LY-0526-LY5812)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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