Cattle CCL8 ELISA Kit (V2LY-0626-LY2499)

Name: Cattle CCL8 ELISA Kit
SKU: V2LY-0626-LY2499
Rating: 5 (1 reviews)

See all CCL8 antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0072 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Cattle

Assay Type

Sandwich

Reactivity

Cattle

Assay Time

1.5 h

Molecule Mass

10.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

C-C Motif Chemokine Ligand 8

Function

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Calcium ion transport Source: UniProtKB
Cell-cell signaling Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: ProtInc
Eosinophil chemotaxis Source: GO_Central
Exocytosis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: GO_Central
Inflammatory response Source: GO_Central
Killing of cells of other organism Source: UniProtKB
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Negative regulation by host of viral genome replication Source: ARUK-UCL
Negative regulation of leukocyte proliferation Source: Ensembl
Neutrophil chemotaxis Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of leukocyte migration Source: Ensembl
Response to virus Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Secreted

PTM

N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Lan, K. F., Shen, Y. Q., Li, Y., Ling, C. L., Gong, Y. M., Xia, S. C., ... & Ding, X. (2021). Chemokine CC motif ligand 8 in periodontal ligament during orthodontic tooth movement. Archives of Oral Biology, 123, 104996.

Amador‐Martínez, I., García‐Ballhaus, J., Buelna‐Chontal, M., Cortés‐González, C., Massó, F., Jaisser, F., & Barrera‐Chimal, J. (2021). Early inflammatory changes and CC chemokine ligand‐8 upregulation in the heart contribute to uremic cardiomyopathy. The FASEB Journal, 35(8), e21761.

Baltaeva, Y. Y., Yuldasheva, М. Т., Palvanova, М. S., & Hoshimov, M. S. (2021). The Specific Role of CCL8 Chemokine in Atherosclerosis. International Journal of Multicultural and Multireligious Understanding, 8(6), 205-211.

Igarashi, K., Hori, T., Yamamoto, M., Sohma, H., Suzuki, N., Tsutsumi, H., ... & Kokai, Y. (2021). CCL8 deficiency in the host abrogates early mortality of acute graft-versus-host disease in mice with dysregulated IL-6 expression. Experimental Hematology.

Xue, S., Tang, H., Zhao, G., Fang, C., Shen, Y., Yan, D., ... & Guo, D. (2021). C–C motif ligand 8 promotes atherosclerosis via NADPH oxidase 2/reactive oxygen species-induced endothelial permeability increase. Free Radical Biology and Medicine, 167, 181-192.

Kim, E. S., Nam, S. M., Song, H. K., Lee, S., Kim, K., Lim, H. K., ... & Moon, A. (2021). CCL8 mediates crosstalk between endothelial colony forming cells and triple-negative breast cancer cells through IL-8, aggravating invasion and tumorigenicity. Oncogene, 40(18), 3245-3259.

Zhang, X., Chen, L., Dang, W. Q., Cao, M. F., Xiao, J. F., Lv, S. Q., ... & Bian, X. W. (2020). CCL8 secreted by tumor-associated macrophages promotes invasion and stemness of glioblastoma cells via ERK1/2 signaling. Laboratory Investigation, 100(4), 619-629.

Dai, S., Zhang, J., & Xu, Z. (2020). Silencing CCL8 inhibited the proliferation and migration of PDGF-BB-stimulated human aortic smooth muscle cells. Bioscience, Biotechnology, and Biochemistry, 84(8), 1585-1593.

Ge, B., Li, J., Wei, Z., Sun, T., Song, Y., & Khan, N. U. (2017). Functional expression of CCL8 and its interaction with chemokine receptor CCR3. BMC immunology, 18(1), 1-8.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Cattle CCL8 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CCL8 protein, His & NusA Tag (CAT#: V2LY-0526-LY8648)

Human Recombinant CCL8 protein, His Tag (CAT#: V2LY-0526-LY5479)

Human Recombinant CCL8 protein, SUMO Tag (CAT#: V2LY-0526-LY5480)

Human CCL8 ELISA Kit (CAT#: V2LY-0626-LY424)

Related Products

Mouse Anti-CCL8 Recombinant Antibody (CBFYM-0961) (CAT#: CBMAB-M1110-FY)

Mouse Anti-CCL8 Recombinant Antibody (CBFYM-0960) (CAT#: CBMAB-M1109-FY)

Human CCL8 ELISA Kit (CAT#: V2LY-0626-LY424)

Human Recombinant CCL8 protein, SUMO Tag (CAT#: V2LY-0526-LY5480)

Mouse Anti-CCL8 Recombinant Antibody (CBFYM-0958) (CAT#: CBMAB-M1107-FY)

Mouse Anti-CCL8 Recombinant Antibody (CBFYM-0959) (CAT#: CBMAB-M1108-FY)

Mouse Recombinant CCL8 protein, His & NusA Tag (CAT#: V2LY-0526-LY8648)

Rat Anti-CCL8 Recombinant Antibody (17A2) (CAT#: CBMAB-C3433-LY)

Human Recombinant CCL8 protein, His Tag (CAT#: V2LY-0526-LY5479)

Mouse Anti-CCL8 Recombinant Antibody (7D27) (CAT#: CBMAB-C8621-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry