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Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0087) (AbAr-0087-YC)

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Specifications

Target Array
Chemokine Array
Target
CCL21, CXCL16, CCL14, IL-28A, LIF, CCL13, CCL23, CXCL4, Axl, CXCL5, CCL16, IL-29, TNFSF14, CCL22, MSP alpha/beta, CXCL12 alpha, Betacellulin, CCL26, IL-9, IL-31, XCL1, MIF, CXCL7, CCL17, CCL28, CXCL6, IL-17F, CXCL10, CCL8, CCL20, SPP1, CCL25, CCL27, GRO, IL-18 BP alpha, CXCL11, CCL7, CCL19, CCL18, TSLP
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids

Applications

Application
Multiplex Protein Detection
Assay Type
Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based
Protocol Outline
Thoroughly dry the glass slide and block the array surface to prevent non-specific binding.
Incubate the array with both samples and standards.
Incubate with a biotinylated detection antibody cocktail and then with streptavidin-conjugated fluorescent dye.
Carefully disassemble the glass slide and scan the slide using a gene microarray laser scanner.
Conduct densitometric analysis.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Chemokines are a family of chemoattractant cytokines (small proteins secreted by cells that influence the immune system) which play a vital role in cell migration through venules from blood into tissue and vice versa, and in the induction of cell movement in response to a chemical (chemokine) gradient by a process known as chemotaxis. In addition, chemokines also regulate lymphoid organ development and T-cell differentiation, mediate tumour cell metastasis, and have recently been shown to have a function in the nervous system as neuromodulators.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008) (CAT#: AbAr-0008-YC)

Mouse Chemokine Antibody Array (Membrane, 25 Targets) (Y0168) (CAT#: AbAr-0168-YC)

Canine Chemokine Antibody Array (Membrane, 9 Targets) (Y0220) (CAT#: AbAr-0220-YC)

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222) (CAT#: AbAr-0222-YC)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241) (CAT#: AbAr-0241-YC)

Rat Chemokine Antibody Array (Membrane, 15 Targets) (Y0410) (CAT#: AbAr-0410-YC)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411) (CAT#: AbAr-0411-YC)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015) (CAT#: AbAr-0015-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109) (CAT#: AbAr-0109-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412) (CAT#: AbAr-0412-YC)

Antibody Array

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008) (CAT#: AbAr-0008-YC)

Mouse Chemokine Antibody Array (Membrane, 25 Targets) (Y0168) (CAT#: AbAr-0168-YC)

Canine Chemokine Antibody Array (Membrane, 9 Targets) (Y0220) (CAT#: AbAr-0220-YC)

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222) (CAT#: AbAr-0222-YC)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241) (CAT#: AbAr-0241-YC)

Rat Chemokine Antibody Array (Membrane, 15 Targets) (Y0410) (CAT#: AbAr-0410-YC)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411) (CAT#: AbAr-0411-YC)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015) (CAT#: AbAr-0015-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109) (CAT#: AbAr-0109-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412) (CAT#: AbAr-0412-YC)

Related Products

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222) (CAT#: AbAr-0222-YC)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241) (CAT#: AbAr-0241-YC)

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008) (CAT#: AbAr-0008-YC)

Mouse Chemokine Antibody Array (Membrane, 25 Targets) (Y0168) (CAT#: AbAr-0168-YC)

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0413) (CAT#: AbAr-0413-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412) (CAT#: AbAr-0412-YC)

Canine Chemokine Antibody Array (Membrane, 9 Targets) (Y0220) (CAT#: AbAr-0220-YC)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109) (CAT#: AbAr-0109-YC)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015) (CAT#: AbAr-0015-YC)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411) (CAT#: AbAr-0411-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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