Mouse Chemokine Antibody Array (Membrane, 25 Targets) (Y0168) (AbAr-0168-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Mouse Chemokine Antibody Array detects 25 Mouse Chemokines. It is suitable for all liquid sample types.
See all Chemokine antibodies

Datasheet

Specifications

Target Array

Chemokine Array

Target

6Ckine (CCL21), Eotaxin-2 (MPIF-2/CCL24), MCP-1 (CCL2), MIP-1 gamma, RANTES (CCL5), BLC (CXCL13), Fractalkine (CX3CL1), MCP-5, MIP-2, SDF-1 (CXCL12), CTACK (CCL27), I-TAC (CXCL11), MDC (CCL22), MIP-3 beta (CCL19), TARC (CCL17), CXCL16, KC (CXCL1), MIG (CXCL9), MIP-3 alpha (CCL20), I-309 (TCA-3/CCL1), Eotaxin-1 (CCL11), LIX, MIP-1 alpha (CCL3), Platelet Factor 4 (CXCL4), TECK (CCL25)

Species Reactivity

Mouse

Sample Type

Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application

Multiplex Protein Detection

Assay Type

Semi-Quantitative

Solid Support

Membrane

Detection Assay

Sandwich-based

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Components

Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction

Chemokines are a family of chemoattractant cytokines (small proteins secreted by cells that influence the immune system) which play a vital role in cell migration through venules from blood into tissue and vice versa, and in the induction of cell movement in response to a chemical (chemokine) gradient by a process known as chemotaxis. In addition, chemokines also regulate lymphoid organ development and T-cell differentiation, mediate tumour cell metastasis, and have recently been shown to have a function in the nervous system as neuromodulators.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Alternative Versions

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109)

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0087)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411)

Rat Chemokine Antibody Array (Membrane, 15 Targets) (Y0410)

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008)

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0413)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241)

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222)

Antibody Array

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008)

Canine Chemokine Antibody Array (Membrane, 9 Targets) (Y0220)

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241)

Rat Chemokine Antibody Array (Membrane, 15 Targets) (Y0410)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015)

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0087)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412)

Related Products

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0413)

Human Chemokine Antibody Array (Glass Slide, 40 Targets) (Y0087)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0109)

Human Chemokine Antibody Array (Glass Slide, 38 Targets) (Y0015)

Canine Chemokine Antibody Array (Membrane, 9 Targets) (Y0220)

Mouse Chemokine Antibody Array (Glass Slide, 25 Targets) (Y0412)

Porcine Chemokine Antibody Array (Membrane, 19 Targets) (Y0411)

Bovine Chemokine Antibody Array (Membrane, 9 Targets) (Y0222)

Human Chemokine Antibody Array (Membrane, 31 Targets) (Y0241)

Human Chemokine Antibody Array (Membrane, 38 Targets) (Y0008)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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