Chicken OCLN ELISA Kit (V2LY-0626-LY572)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.024 ng/mL
Detection Range
0.05-30 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Chicken
Assay Type
Sandwich
Reactivity
Chicken
Assay Time
1.5 h
Molecule Mass
55.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Occludin
Function
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
(Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Biological Process
Bicellular tight junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Cell-cell junction organizationManual Assertion Based On ExperimentIMP:MGI
Maintenance of blood-brain barrier1 PublicationNAS:ARUK-UCL
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of blood-brain barrier permeabilityManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of glucose importManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of lamellipodium assemblyISS:ARUK-UCL
Positive regulation of microtubule polymerizationISS:ARUK-UCL
Positive regulation of wound healingISS:ARUK-UCL
Protein localization to cell leading edgeISS:ARUK-UCL
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Regulation of glucose transmembrane transportManual Assertion Based On ExperimentIMP:ARUK-UCL
Cellular Location
Cell membrane
Cell junction, tight junction
Involvement in disease
Pseudo-TORCH syndrome 1 (PTORCH1):
An autosomal recessive neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
Topology
Cytoplasmic: 1-66
Helical: 67-89
Extracellular: 90-135
Helical: 136-160
Cytoplasmic: 161-170
Helical: 171-195
Extracellular: 196-243
Helical: 244-265
Cytoplasmic: 266-522
PTM
Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

Zhang, J., Yang, W., Roy, S., Liu, H., Roberts, R. M., Wang, L., ... & Ma, W. (2023). Tight junction protein occludin is an internalization factor for SARS-CoV-2 infection and mediates virus cell-to-cell transmission. Proceedings of the National Academy of Sciences, 120(17), e2218623120.

Kuo, W. T., Odenwald, M. A., Turner, J. R., & Zuo, L. (2022). Tight junction proteins occludin and ZO‐1 as regulators of epithelial proliferation and survival. Annals of the New York Academy of Sciences, 1514(1), 21-33.

Yang, F., Liu, X. Q., He, J. Z., Xian, S. P., Yang, P. F., Mai, Z. Y., ... & Zhang, X. D. (2022). Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4. Journal of Cellular and Molecular Medicine, 26(8), 2363-2376.

Hou, Q., Huang, Y., Wang, Y., Liao, L., Zhu, Z., Zhang, W., ... & Liu, F. (2020). Lactobacillus casei LC01 regulates intestinal epithelial permeability through miR-144 targeting of OCLN and ZO1. Journal of Microbiology and Biotechnology, 30(10), 1480.

Zhou, T., Lu, Y., Xu, C., Wang, R., Zhang, L., & Lu, P. (2020). Occludin protects secretory cells from ER stress by facilitating SNARE-dependent apical protein exocytosis. Proceedings of the National Academy of Sciences, 117(9), 4758-4769.

Lavie, M., Linna, L., Moustafa, R. I., Belouzard, S., Fukasawa, M., & Dubuisson, J. (2019). Role of the cytosolic domain of occludin in trafficking and hepatitis C virus infection. Traffic, 20(10), 753-773.

Bendriem, R. M., Singh, S., Aleem, A. A., Antonetti, D. A., & Ross, M. E. (2019). Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. Elife, 8, e49376.

Shimizu, Y., Shirasago, Y., Suzuki, T., Hata, T., Kondoh, M., Hanada, K., ... & Fukasawa, M. (2019). Characterization of monoclonal antibodies recognizing each extracellular loop domain of occludin. The journal of biochemistry, 166(4), 297-308.

Lavie, M., Linna, L., Moustafa, R. I., Belouzard, S., Fukasawa, M., & Dubuisson, J. (2019). Role of the cytosolic domain of occludin in trafficking and HCV infection 1 Running Title: OCLN C-terminus and HCV entry 2. Traffic.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Dog OCLN ELISA Kit (CAT#: V2LY-0626-LY147)

Rat Ocln ELISA Kit (CAT#: V2LY-0626-LY2476)

Mouse Ocln ELISA Kit (CAT#: V2LY-0626-LY3270)

Human OCLN ELISA Kit (CAT#: V2LY-0626-LY3909)

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Mouse Anti-OCLN Recombinant Antibody (1G7) (CAT#: CBMAB-O0249-CQ)

Mouse Ocln ELISA Kit (CAT#: V2LY-0626-LY3270)

Mouse Anti-OCLN (AA 423-523) Recombinant Antibody (CBXO-0544) (CAT#: CBMAB-O0248-CQ)

Mouse Anti-OCLN (AA 396-507) Recombinant Antibody (CBXO-0535) (CAT#: CBMAB-O0012-CQ)

Rabbit Anti-OCLN (AA 387-403) Recombinant Antibody (CBXO-0446) (CAT#: CBMAB-O0238-CQ)

Mouse Anti-OCLN (C-Terminus) Recombinant Antibody (OC-3F10) (CAT#: CBMAB-O0067-CQ)

Rat Ocln ELISA Kit (CAT#: V2LY-0626-LY2476)

Isotype control

For research use only. Not intended for any clinical use.

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