Mouse Anti-OCLN Recombinant Antibody (CBXO-0403) (CBMAB-O0239-CQ)

Name: Mouse Anti-OCLN Recombinant Antibody (CBXO-0403)
SKU: CBMAB-O0239-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXO-0403

Application

ELISA, WB, IHC

Specificity

Human, Mouse, Pig, Rat

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Occludin

Introduction

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Entrez Gene ID

Human	100506658
Mouse	18260
Rat	83497
Pig	397236

UniProt ID

Human	Q16625
Mouse	Q61146
Rat	Q6P6T5
Pig	A0A287AL69

Alternative Names

Occludin; Phosphatase 1, Regulatory Subunit 115; Tight Junction Protein Occludin TM4 Minus; Tight Junction Protein Occludin; PPP1R115; PTORCH1; BLCPMG

Function

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
(Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

Biological Process

Bicellular tight junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Cell-cell junction organizationManual Assertion Based On ExperimentIMP:MGI
Maintenance of blood-brain barrier1 PublicationNAS:ARUK-UCL
Negative regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of protein phosphorylationManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of blood-brain barrier permeabilityManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of gene expressionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of glucose importManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of lamellipodium assemblyISS:ARUK-UCL
Positive regulation of microtubule polymerizationISS:ARUK-UCL
Positive regulation of wound healingISS:ARUK-UCL
Protein localization to cell leading edgeISS:ARUK-UCL
Protein-containing complex assemblyManual Assertion Based On ExperimentTAS:ProtInc
Regulation of glucose transmembrane transportManual Assertion Based On ExperimentIMP:ARUK-UCL

Cellular Location

Cell membrane
Cell junction, tight junction

Involvement in disease

Pseudo-TORCH syndrome 1 (PTORCH1):
An autosomal recessive neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.

Topology

Cytoplasmic: 1-66
Helical: 67-89
Extracellular: 90-135
Helical: 136-160
Cytoplasmic: 161-170
Helical: 171-195
Extracellular: 196-243
Helical: 244-265
Cytoplasmic: 266-522

PTM

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

Zhang, J., Yang, W., Roy, S., Liu, H., Roberts, R. M., Wang, L., ... & Ma, W. (2023). Tight junction protein occludin is an internalization factor for SARS-CoV-2 infection and mediates virus cell-to-cell transmission. Proceedings of the National Academy of Sciences, 120(17), e2218623120.

Kuo, W. T., Odenwald, M. A., Turner, J. R., & Zuo, L. (2022). Tight junction proteins occludin and ZO‐1 as regulators of epithelial proliferation and survival. Annals of the New York Academy of Sciences, 1514(1), 21-33.

Yang, F., Liu, X. Q., He, J. Z., Xian, S. P., Yang, P. F., Mai, Z. Y., ... & Zhang, X. D. (2022). Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4. Journal of Cellular and Molecular Medicine, 26(8), 2363-2376.

Hou, Q., Huang, Y., Wang, Y., Liao, L., Zhu, Z., Zhang, W., ... & Liu, F. (2020). Lactobacillus casei LC01 regulates intestinal epithelial permeability through miR-144 targeting of OCLN and ZO1. Journal of Microbiology and Biotechnology, 30(10), 1480.

Zhou, T., Lu, Y., Xu, C., Wang, R., Zhang, L., & Lu, P. (2020). Occludin protects secretory cells from ER stress by facilitating SNARE-dependent apical protein exocytosis. Proceedings of the National Academy of Sciences, 117(9), 4758-4769.

Lavie, M., Linna, L., Moustafa, R. I., Belouzard, S., Fukasawa, M., & Dubuisson, J. (2019). Role of the cytosolic domain of occludin in trafficking and hepatitis C virus infection. Traffic, 20(10), 753-773.

Bendriem, R. M., Singh, S., Aleem, A. A., Antonetti, D. A., & Ross, M. E. (2019). Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. Elife, 8, e49376.

Shimizu, Y., Shirasago, Y., Suzuki, T., Hata, T., Kondoh, M., Hanada, K., ... & Fukasawa, M. (2019). Characterization of monoclonal antibodies recognizing each extracellular loop domain of occludin. The journal of biochemistry, 166(4), 297-308.

Lavie, M., Linna, L., Moustafa, R. I., Belouzard, S., Fukasawa, M., & Dubuisson, J. (2019). Role of the cytosolic domain of occludin in trafficking and HCV infection 1 Running Title: OCLN C-terminus and HCV entry 2. Traffic.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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