Cynomolgus Recombinant CA4 protein, Biotin Conjugated, His & AVI Tag (V2LY-0526-LY1400)

Name: Cynomolgus Recombinant CA4 protein, Biotin Conjugated, His & AVI Tag
SKU: V2LY-0526-LY1400
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

His & AVI Tag

Protein Construction

This product is Cynomolgus Recombinant CA4 protein, Biotin Conjugated, His & AVI Tag consist of Amino Acid: 1-286 and predicts a molecular mass of 33.44 kDa.

Molecule Mass

33.44 kDa

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-286

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Carbonic Anhydrase 4

Function

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Biological Process

Bicarbonate transport Source: DFLAT
One-carbon metabolic process Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Retinitis pigmentosa 17 (RP17): The disease is caused by variants affecting the gene represented in this entry. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

Xu, Y., Xu, W. H., Shi, S. N., Yang, X. L., Ren, Y. R., Zhuang, X. Y., ... & Zhang, X. F. (2020). Carbonic Anhydrase 4 serves as a Clinicopathological Biomarker for Outcomes and Immune Infiltration in Renal Cell Carcinoma, Lower Grade Glioma, Lung Adenocarcinoma and Uveal Melanoma. Journal of Cancer, 11(20), 6101.

Rudenko, N. N., Fedorchuk, T. P., Terentyev, V. V., Dymova, O. V., Naydov, I. A., Golovko, T. K., ... & Ivanov, B. N. (2020). The role of carbonic anhydrase α-CA4 in the adaptive reactions of photosynthetic apparatus: the study with α-CA4 knockout plants. Protoplasma, 257(2), 489-499.

Wang, B., Jiang, H., Wan, X., Wang, Y., Zheng, X., Li, P., ... & Song, H. (2020). Carbonic anhydrase IV inhibits cell proliferation in gastric cancer by regulating the cell cycle. Oncology Letters, 20(4), 1-1.

Zhou, Y., Vroegop-Vos, I. A., Van Dijken, A. J., Van der Does, D., Zipfel, C., Pieterse, C. M., & Van Wees, S. C. (2020). Carbonic anhydrases CA1 and CA4 function in atmospheric CO 2-modulated disease resistance. Planta, 251(4), 1-14.

Chew, S. F., Koh, C. Z., Hiong, K. C., Choo, C. Y., Wong, W. P., Neo, M. L., & Ip, Y. K. (2019). Light-enhanced expression of Carbonic Anhydrase 4-like supports shell formation in the fluted giant clam Tridacna squamosa. Gene, 683, 101-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CA4 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2552)

Human Recombinant CA4 protein, His Tag (CAT#: V2LY-0526-LY2553)

Mouse Recombinant CA4 protein, His Tag (CAT#: V2LY-0526-LY7906)

Related Products

Human Recombinant CA4 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2552)

Mouse Recombinant CA4 protein, His Tag (CAT#: V2LY-0526-LY7906)

Human Recombinant CA4 protein, His Tag (CAT#: V2LY-0526-LY2553)

Mouse Anti-CA4 Recombinant Antibody (CBFYC-0696) (CAT#: CBMAB-C0750-FY)

Mouse Anti-CA4 Recombinant Antibody (CBYY-C0002) (CAT#: CBMAB-C0007-YY)

Rabbit Anti-CA4 Recombinant Antibody (039) (CAT#: CBMAB-C1732-LY)

Mouse Anti-CA4 Recombinant Antibody (310415) (CAT#: CBMAB-C0944-LY)

Mouse Anti-CA4 Recombinant Antibody (4G6) (CAT#: CBMAB-C7004-LY)

Mouse Anti-CA4 Recombinant Antibody (310413) (CAT#: CBMAB-C5476-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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