Cynomolgus Recombinant FAP protein, ECD, His Tag (V2LY-0526-LY1508)

Name: Cynomolgus Recombinant FAP protein, ECD, His Tag
SKU: V2LY-0526-LY1508
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

His Tag

Protein Construction

This product is Cynomolgus Recombinant FAP protein, ECD, His Tag consist of Amino Acid: 30-760 and predicts a molecular mass of 87 kDa.

Molecule Mass

87 kDa

Protein Domain

ECD

Sequence

Amino Acid: 30-760

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Fibroblast Activation Protein Alpha

Research Area

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16480718, PubMed:16410248, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721).

Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:9065413, PubMed:2172980, PubMed:7923219, PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711).

Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16651416, PubMed:16410248, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288).

Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817).

The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

Biological Process

Angiogenesis Source: UniProtKB-KW
Cell adhesion Source: UniProtKB-KW
Endothelial cell migration Source: UniProtKB
Melanocyte apoptotic process Source: UniProtKB
Melanocyte proliferation Source: UniProtKB
Negative regulation of cell proliferation involved in contact inhibition Source: UniProtKB
Negative regulation of extracellular matrix disassembly Source: UniProtKB
Negative regulation of extracellular matrix organization Source: UniProtKB
Positive regulation of execution phase of apoptosis Source: UniProtKB
Proteolysis Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of collagen catabolic process Source: UniProtKB
Regulation of fibrinolysis Source: BHF-UCL

Cellular Location

Prolyl endopeptidase FAP: Cell membrane; Lamellipodium membrane; Invadopodium membrane; Ruffle membrane; Cell surface; Membrane. Localized on cell surface with lamellipodia and invadopodia membranes and on shed vesicles. Colocalized with DPP4 at invadopodia and lamellipodia membranes of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Anchored and enriched preferentially by integrin alpha-3/beta-1 at invadopodia, plasma membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner. Colocalized with PLAUR preferentially at the cell surface of invadopodia membranes in a cytoskeleton-, integrin- and vitronectin-dependent manner. Concentrated at invadopodia membranes, specialized protrusions of the ventral plasma membrane in a fibrobectin-dependent manner. Colocalizes with extracellular components (ECM), such as collagen fibers and fibronectin.
Antiplasmin-cleaving enzyme FAP, soluble form: Secreted. Found in blood plasma and serum.
Isoform 2: Cytoplasm

Topology

Cytoplasmic: 1-4
Helical: 5-25
Extracellular: 26-760

PTM

N-glycosylated.
The N-terminus may be blocked.

Yuan, Z., Hu, H., Zhu, Y., Zhang, W., Fang, Q., Qiao, T., ... & Wang, X. (2021). Colorectal cancer cell intrinsic fibroblast activation protein alpha binds to Enolase1 and activates NF-κB pathway to promote metastasis. Cell death & disease, 12(6), 1-15.

Labiano, S., Roh, V., Godfroid, C., Hiou-Feige, A., Romero, J., Sum, E., ... & Romero, P. (2021). CD40 Agonist Targeted to Fibroblast Activation Protein α Synergizes with Radiotherapy in Murine HPV-Positive Head and Neck TumorsSynergistic Antitumor Effect of Radiotherapy and FAP-CD40 in HNSCC. Clinical Cancer Research, 27(14), 4054-4065.

Juillerat-Jeanneret, L., Tafelmeyer, P., & Golshayan, D. (2021). Regulation of fibroblast activation protein-α expression: focus on intracellular protein interactions. Journal of Medicinal Chemistry, 64(19), 14028-14045.

Bughda, R., Dimou, P., D’Souza, R. R., & Klampatsa, A. (2021). Fibroblast activation protein (FAP)-targeted CAR-T cells: Launching an attack on tumor stroma. ImmunoTargets and Therapy, 10, 313.

Fitzgerald, A. A., & Weiner, L. M. (2020). The role of fibroblast activation protein in health and malignancy. Cancer and Metastasis Reviews, 39(3), 783-803.

Hintz, H. M., Gallant, J. P., Vander Griend, D. J., Coleman, I. M., Nelson, P. S., & LeBeau, A. M. (2020). Imaging Fibroblast Activation Protein Alpha Improves Diagnosis of Metastatic Prostate Cancer with Positron Emission TomographyImaging FAP in Prostate Cancer. Clinical Cancer Research, 26(18), 4882-4891.

Roy, J., Hettiarachchi, S. U., Kaake, M., Mukkamala, R., & Low, P. S. (2020). Design and validation of fibroblast activation protein alpha targeted imaging and therapeutic agents. Theranostics, 10(13), 5778-5789.

Solano-Iturri, J. D., Beitia, M., Errarte, P., Calvete-Candenas, J., Etxezarraga, M. C., Loizate, A., ... & Larrinaga, G. (2020). Altered expression of fibroblast activation protein-α (FAP) in colorectal adenoma-carcinoma sequence and in lymph node and liver metastases. Aging (Albany NY), 12(11), 10337.

Windisch, P., Röhrich, M., Regnery, S., Tonndorf-Martini, E., Held, T., Lang, K., ... & Adeberg, S. (2020). Fibroblast activation protein (FAP) specific PET for advanced target volume delineation in glioblastoma. Radiotherapy and Oncology, 150, 159-163.

Solano-Iturri, J. D., Errarte, P., Etxezarraga, M. C., Echevarria, E., Angulo, J., López, J. I., & Larrinaga, G. (2020). Altered tissue and plasma levels of fibroblast activation protein-α (FAP) in renal tumours. Cancers, 12(11), 3393.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

FAP Matched Antibody Pair (409) (CAT#: APMAB-409LY)

Human Recombinant FAP protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4018)

Human Recombinant FAP protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY4019)

Human Recombinant FAP protein, ECD, His Tag (CAT#: V2LY-0526-LY4020)

Human Recombinant FAP protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY4021)

Human Recombinant FAP protein, ECD, His Tag-2 (CAT#: V2LY-0526-LY4022)

Human Recombinant FAP protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY4023)

Mouse Recombinant FAP protein, hFc Tag (CAT#: V2LY-0526-LY8296)

Antibody Pairs

FAP Matched Antibody Pair (409) (CAT#: APMAB-409LY)

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Human Recombinant FAP protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4018)

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Human Recombinant FAP protein, ECD, His Tag (CAT#: V2LY-0526-LY4020)

Human Recombinant FAP protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY4023)

Human Recombinant FAP protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY4019)

Human Recombinant FAP protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY4021)

Human Recombinant FAP protein, ECD, His Tag-2 (CAT#: V2LY-0526-LY4022)

Mouse Anti-FAP Recombinant Antibody (CBXF-1919) (CAT#: V2LY-0825-LY1387)

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For research use only. Not intended for any clinical use.

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