Cynomolgus Recombinant IGFBP3 protein, His Tag (V2LY-0526-LY1542)

Name: Cynomolgus Recombinant IGFBP3 protein, His Tag
SKU: V2LY-0526-LY1542
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cynomolgus

Tag

His Tag

Protein Construction

This product is Cynomolgus Recombinant IGFBP3 protein, His Tag consist of Amino Acid: 28-291 and predicts a molecular mass of 31.1 kDa.

Molecule Mass

31.1 kDa

Verified

HPLC

Sequence

Amino Acid: 28-291

Species

Cynomolgus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Insulin Like Growth Factor Binding Protein 3

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253).

Promotes testicular germ cell apoptosis (PubMed:19952275).

Biological Process

Apoptotic process Source: UniProtKB-KW
Negative regulation of cell population proliferation Source: MGI
Negative regulation of protein phosphorylation Source: MGI
Negative regulation of signal transduction Source: UniProtKB
Negative regulation of smooth muscle cell migration Source: BHF-UCL
Negative regulation of smooth muscle cell proliferation Source: BHF-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of myoblast differentiation Source: UniProtKB
Protein phosphorylation Source: MGI
Regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central

Cellular Location

Secreted

PTM

Phosphorylated by FAM20C in the extracellular medium.

Xiao, Z., Xie, Y., Huang, F., Yang, J., Liu, X., Lin, X., ... & Zheng, S. (2022). MicroRNA-205-5p plays a suppressive role in the high-fat diet-induced atrial fibrosis through regulation of the EHMT2/IGFBP3 axis. Genes & Nutrition, 17(1), 1-17.

Yang, J., He, Q., Wang, Y., Pan, Z., Zhang, G., Liang, J., ... & Chen, P. (2022). Gegen Qinlian Decoction ameliorates type 2 diabetes osteoporosis via IGFBP3/MAPK/NFATc1 signaling pathway based on cytokine antibody array. Phytomedicine, 94, 153810.

Almalki, A., Thomas, J. T., Khan, A. R. A., Almulhim, B., Alassaf, A., Alghamdi, S. A., ... & Alotaibi, S. (2022). Correlation between Salivary Levels of IGF-1, IGFBP-3, IGF-1/IGFBP3 Ratio with Skeletal Maturity Using Hand-Wrist Radiographs. International Journal of Environmental Research and Public Health, 19(6), 3723.

D’Addio, F., Maestroni, A., Assi, E., Ben Nasr, M., Amabile, G., Usuelli, V., ... & Fiorina, P. (2022). The IGFBP3/TMEM219 pathway regulates beta cell homeostasis. Nature communications, 13(1), 684.

Jin, L., Shen, F., Weinfeld, M., & Sergi, C. (2020). Insulin growth factor binding protein 7 (IGFBP7)-related cancer and IGFBP3 and IGFBP7 crosstalk. Frontiers in Oncology, 10, 727.

Long, Z., Gong, F., Li, Y., Fan, Z., & Li, J. (2020). Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis. Cancer cell international, 20, 1-11.

Li, C. L., Liu, B., Wang, Z. Y., Xie, F., Qiao, W., Cheng, J., ... & Liu, D. S. (2020). Salvianolic acid B improves myocardial function in diabetic cardiomyopathy by suppressing IGFBP3. Journal of molecular and cellular cardiology, 139, 98-112.

Yin, L., Chen, J., Ma, C., Pei, S., Du, M., Zhang, Y., ... & Feng, J. (2020). Hsa_circ_0046263 functions as a ceRNA to promote nasopharyngeal carcinoma progression by upregulating IGFBP3. Cell Death & Disease, 11(7), 562.

Xiong, L. L., Xue, L. L., Du, R. L., Zhou, H. L., Tan, Y. X., Ma, Z., ... & Wang, T. H. (2020). Vi4-miR-185-5p-Igfbp3 network protects the brain from neonatal hypoxic ischemic injury via promoting neuron survival and suppressing the cell apoptosis. Frontiers in Cell and Developmental Biology, 8, 529544.

Chen, C. H., Chen, P. Y., Lin, Y. Y., Feng, L. Y., Chen, S. H., Chen, C. Y., ... & Wei, K. C. (2019). Suppression of tumor growth via IGFBP3 depletion as a potential treatment in glioma. Journal of Neurosurgery, 132(1), 168-179.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IGFBP3 protein, His Tag (CAT#: V2LY-0526-LY4669)

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Isotype control

For research use only. Not intended for any clinical use.

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