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Human Cytokine Antibody Array (Glass Slide, 280 Targets) (Y0479) (AbAr-0479-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Cytokine Antibody Array, which can be used for semi-quantitative detection of 280 human cytokines. The antibody array product is a combination of 7 non-overlapping arrays. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Cytokine antibodies

Specifications

Target Array
Cytokine Array
Target
2B4 (CD244), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), Activin A, ADAM-9, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, ALCAM (CD166), Alpha-fetoprotein, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiostatin, ANGPTL4, APRIL, AXL, BCAM, BCMA, BDNF, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-9, C5a, CA125, CA15-3, Cathepsin L, Cathepsin S, CCL28 (MEC), CD14, CD200, CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD80 (B7-1), CD97, CEA (CEACAM-5), CEACAM-1, Chemerin, Contactin-2 (TAG1), Cripto-1, CRP (C-Reactive Protein), CTACK (CCL27), CXCL16, DAN, DcR3, DKK-1, DR6 (TNFRSF21), Dtk, E-Cadherin, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB2, ErbB3, E-Selectin, FABP2, FAP, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), Fc gamma RIIB/C (CD32B/C), Ferritin, FGF-19, FGF-4, FGF-7 (KGF), Flt-3 Ligand, Follistatin, FSH, Galectin-3, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, HGF, HGFR, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-alpha / beta R2, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-16, IL-17 RA, IL-17A, IL-17B, IL-17F, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-21, IL-21 R, IL-23, IL-24, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-33 (IL-1 F11), IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), Kallikrein 14, LAP/TGF beta 1 (TGF beta 1), Legumain, Leptin, LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), LOX-1, L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), Neprilysin, NGFR (TNFRSF16), Nidogen-1, Notch-1, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Oncostatin M, Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PD-1, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PGRPs, Platelet Factor 4 (CXCL4), PLGF, Procalcitonin, Prolactin, PSA-free, RAGE, RANTES (CCL5), Resistin, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), SERPINA4, sFRP-3, Siglec-5 (CD170), Siglec-9, Sonic Hedgehog N-Terminal (Shh-N), TACE, TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombomodulin, Thrombopoietin (TPO), Thyroglobulin, Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TIMP-4, TLR2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Transferrin, Trappin-2, TREM-1, TROP1 (EpCAM), TSH, TSLP, uPAR, VCAM-1 (CD106), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, XEDAR
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Dry the array glass slide
Block glass slide surface
Incubate with Samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Cytokines are a broad and loose category of small proteins (5–20 kDa) that are important in cell signaling. Cytokines are peptides, and cannot cross the lipid bilayer of cells to enter the cytoplasm. Cytokines have been shown to be involved in autocrine signaling, paracrine signaling and endocrine signaling as immunomodulating agents. Their definite distinction from hormones is still part of ongoing research. Cytokines include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced by more than one type of cell.
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Alternative Versions

Human Cytokine Antibody Array (Glass Slide, 274 Targets) (Y0036)

Human Cytokine Antibody Array (Glass Slide, 80 Targets) (Y0037)

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Mouse Cytokine Antibody Array (Membrane, 97 Targets) (Y0066)

Mouse Cytokine Antibody Array (Membrane, 34 Targets) (Y0064)

Mouse Cytokine Antibody Array (Membrane, 32 Targets) (Y0062)

Mouse Cytokine Antibody Array (Membrane, 144 Targets) (Y0061)

Mouse Cytokine Antibody Array (Glass Slide, 144 Targets) (Y0069)

Mouse Cytokine Antibody Array (Membrane, 22 Targets) (Y0060)

Mouse Cytokine Antibody Array (Membrane, 96 Targets) (Y0059)

Antibody Array

Human Cytokine Antibody Array (Membrane, 120 Targets) (Y0020)

Human Cytokine Antibody Array (Membrane, 49 Targets) (Y0021)

Canine Cytokine Antibody Array (Membrane, 40 Targets) (Y0022)

Human Cytokine Antibody Array (Membrane, 174 Targets) (Y0023)

Human Cytokine Antibody Array (Membrane, 42 Targets) (Y0024)

Human Cytokine Antibody Array (Membrane, 274 Targets) (Y0025)

Human Cytokine Antibody Array (Membrane, 36 Targets) (Y0026)

Human Cytokine Antibody Array (Membrane, 80 Targets) (Y0027)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0028)

Human Cytokine Antibody Array (Membrane, 60 Targets) (Y0029)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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