Anti-ERG Antibody (CBMAB-E1756-FY) Immunohistochemistry Kit (V2LY-0826-LY185)

Name: Anti-ERG Antibody (CBMAB-E1756-FY) Immunohistochemistry Kit
SKU: V2LY-0826-LY185
Rating: 5 (1 reviews)

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Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific mouse primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

CBMAB-E1756-FY

Primary Antibody

Rabbit Anti-ERBB2 Recombinant Antibody (8)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Mouse

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated mouse primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Mouse Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml	Inquiry
LV02	Washing Buffer (30×)	120 ml	Inquiry
LV03	Blocking Buffer	5 ml	Inquiry
LV04	Quenching buffer (RTU)	5 ml	Inquiry
LV05	Primary Antibody Diluent (RTU)	100 ml	Inquiry
LV06M	HRP-Goat anti-Mouse Secondary Antibody	5 ml	Inquiry
LV07	Chromogen Component A	0.2 ml	Inquiry
LV08	Chromogen Component B	4 ml	Inquiry
LV09	Signal Enhancer	5 ml	Inquiry
LV10	Counter Staining Reagent	5 ml	Inquiry
LV11	Mounting Media	5 ml	Inquiry

Gomez-Salinero, J. M., Itkin, T., Houghton, S., Badwe, C., Lin, Y., Kalna, V., ... & Rafii, S. (2022). Cooperative ETS transcription factors enforce adult endothelial cell fate and cardiovascular homeostasis. Nature Cardiovascular Research, 1-18.

Khosh Kish, E., Choudhry, M., Gamallat, Y., Buharideen, S. M., & Bismar, T. A. (2022). The Expression of Proto-Oncogene ETS-Related Gene (ERG) Plays a Central Role in the Oncogenic Mechanism Involved in the Development and Progression of Prostate Cancer. International Journal of Molecular Sciences, 23(9), 4772.

Masamoto, Y., Chiba, A., Takezaki, T., Hino, T., Hayashida, H., Mizuno, H., ... & Kurokawa, M. (2021). EVI1-Positive AML Cells Show Distinct Features and High Dependency on ETS Transcription Factor ERG. Blood, 138, 784.

Saulnier, O., Guedri-Idjouadiene, K., Aynaud, M. M., Chakraborty, A., Bruyr, J., Pineau, J., ... & Dequiedt, F. (2021). ERG transcription factors have a splicing regulatory function involving RBFOX2 that is altered in the EWS-FLI1 oncogenic fusion. Nucleic acids research, 49(9), 5038-5056.

Wasmuth, E. V., Hoover, E. A., Antar, A., Klinge, S., Chen, Y., & Sawyers, C. L. (2020). Modulation of androgen receptor DNA binding activity through direct interaction with the ETS transcription factor ERG. Proceedings of the National Academy of Sciences, 117(15), 8584-8592.

Hsing, M., Wang, Y., Rennie, P. S., Cox, M. E., & Cherkasov, A. (2020). ETS transcription factors as emerging drug targets in cancer. Medicinal Research Reviews, 40(1), 413-430.

Kalna, V., Yang, Y., Peghaire, C. R., Frudd, K., Hannah, R., Shah, A. V., ... & Birdsey, G. M. (2019). The transcription factor ERG regulates super-enhancers associated with an endothelial-specific gene expression program. Circulation research, 124(9), 1337-1349.

Nicholas, T. R., Strittmatter, B. G., & Hollenhorst, P. C. (2019). Oncogenic ETS factors in prostate cancer. Prostate Cancer, 409-436.

Sandoval, G. J., Pulice, J. L., Pakula, H., Schenone, M., Takeda, D. Y., Pop, M., ... & Kadoch, C. (2018). Binding of TMPRSS2-ERG to BAF chromatin remodeling complexes mediates prostate oncogenesis. Molecular cell, 71(4), 554-566.

Xu, Z., Wang, Y., Xiao, Z. G., Zou, C., Zhang, X., Wang, Z., ... & Chan, F. L. (2018). Nuclear receptor ERRα and transcription factor ERG form a reciprocal loop in the regulation of TMPRSS2: ERG fusion gene in prostate cancer. Oncogene, 37(48), 6259-6274.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Primary Antibody

Mouse Anti-ERG Recombinant Antibody (CBFYE-1186) (CAT#: CBMAB-E1756-FY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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