Rabbit Anti-ERG Recombinant Antibody (R0036) (V2LY-0825-LY703)

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Basic Information

Host Animal
Rabbit
Clone
R0036
Application
WB, IHC-P, IF, IP
Immunogen
Human ERG.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000-1:2,000
IP1:20-1:50
IF(ICC)1:50-1:200
IHC-P1:100-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, glycerol, BSA
Preservative
Sodium azide
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ERG, ETS Transcription Factor
Entrez Gene ID
Human2078
Mouse13876
Rat170909
Hamster101823381
Pig100625364
Guinea pig100713723
Horse100051563
UniProt ID
HumanP11308
MouseP81270
HamsterA0A1U8BWG6
PigI3L830
Guinea pigH0VA82
HorseA0A3Q2H4T3
Research Area
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Biological Process
Cell differentiation Source: GO_Central
Multicellular organism development Source: ProtInc
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Protein phosphorylation Source: ProtInc
Regulation of transcription by RNA polymerase II Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Cytoplasm; Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Involvement in disease
Ewing sarcoma (ES):
The gene represented in this entry is involved in disease pathogenesis. A chromosomal aberration involving ERG has been found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors.
Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS (PubMed:8187069). Translocation t(X;21)(q25-26;q22) with ELF4 (PubMed:16303180).

Gomez-Salinero, J. M., Itkin, T., Houghton, S., Badwe, C., Lin, Y., Kalna, V., ... & Rafii, S. (2022). Cooperative ETS transcription factors enforce adult endothelial cell fate and cardiovascular homeostasis. Nature Cardiovascular Research, 1-18.

Khosh Kish, E., Choudhry, M., Gamallat, Y., Buharideen, S. M., & Bismar, T. A. (2022). The Expression of Proto-Oncogene ETS-Related Gene (ERG) Plays a Central Role in the Oncogenic Mechanism Involved in the Development and Progression of Prostate Cancer. International Journal of Molecular Sciences, 23(9), 4772.

Masamoto, Y., Chiba, A., Takezaki, T., Hino, T., Hayashida, H., Mizuno, H., ... & Kurokawa, M. (2021). EVI1-Positive AML Cells Show Distinct Features and High Dependency on ETS Transcription Factor ERG. Blood, 138, 784.

Saulnier, O., Guedri-Idjouadiene, K., Aynaud, M. M., Chakraborty, A., Bruyr, J., Pineau, J., ... & Dequiedt, F. (2021). ERG transcription factors have a splicing regulatory function involving RBFOX2 that is altered in the EWS-FLI1 oncogenic fusion. Nucleic acids research, 49(9), 5038-5056.

Wasmuth, E. V., Hoover, E. A., Antar, A., Klinge, S., Chen, Y., & Sawyers, C. L. (2020). Modulation of androgen receptor DNA binding activity through direct interaction with the ETS transcription factor ERG. Proceedings of the National Academy of Sciences, 117(15), 8584-8592.

Hsing, M., Wang, Y., Rennie, P. S., Cox, M. E., & Cherkasov, A. (2020). ETS transcription factors as emerging drug targets in cancer. Medicinal Research Reviews, 40(1), 413-430.

Kalna, V., Yang, Y., Peghaire, C. R., Frudd, K., Hannah, R., Shah, A. V., ... & Birdsey, G. M. (2019). The transcription factor ERG regulates super-enhancers associated with an endothelial-specific gene expression program. Circulation research, 124(9), 1337-1349.

Nicholas, T. R., Strittmatter, B. G., & Hollenhorst, P. C. (2019). Oncogenic ETS factors in prostate cancer. Prostate Cancer, 409-436.

Sandoval, G. J., Pulice, J. L., Pakula, H., Schenone, M., Takeda, D. Y., Pop, M., ... & Kadoch, C. (2018). Binding of TMPRSS2-ERG to BAF chromatin remodeling complexes mediates prostate oncogenesis. Molecular cell, 71(4), 554-566.

Xu, Z., Wang, Y., Xiao, Z. G., Zou, C., Zhang, X., Wang, Z., ... & Chan, F. L. (2018). Nuclear receptor ERRα and transcription factor ERG form a reciprocal loop in the regulation of TMPRSS2: ERG fusion gene in prostate cancer. Oncogene, 37(48), 6259-6274.

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For research use only. Not intended for any clinical use.

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