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Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539) (AbAr-0539-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Growth Factor Antibody Array, which can be used for semi-quantitative detection of 40 human growth factor. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
Specifications
Target Array
Growth Factor Array
Target
Amphiregulin, BDNF, bFGF, BMP-4, BMP-5, BMP-7, beta-NGF, EGF, EGFR, EG-VEGF(PK1), FGF-4, FGF-7(KGF), GDF-15, GDNF, Growth Hormone, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, Insulin, M-CSF R, NGFR(TNFRSF16), NT-3, NT-4, Osteoprotegerin(TNFRSF11B), PDGF-AA, PLGF, SCF, SCF R(CD117/c-kit), TGF alpha, TGF beta 1, TGF beta 3, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates
Applications
Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Dry the array glass slide
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.
Background
Introduction
A growth factor is a naturally occurring substance capable of stimulating cellular growth, proliferation, healing, and cellular differentiation. Usually it is a protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes. Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells. They often promote cell differentiation and maturation, which varies between growth factors. For example, epidermal growth factor (EGF) enhances osteogenic differentiation, while fibroblast growth factors and vascular endothelial growth factors stimulate blood vessel differentiation (angiogenesis).
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For Research Use Only. Not For Clinical Use.