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Rat Growth Factor Antibody Array (Membrane, 14 Targets) (Y0538) (AbAr-0538-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Rat Growth Factor Antibody Array, which can be used for semi-quantitative detection of 14 rat growth factor. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Growth Factor antibodies

Specifications

Target Array
Growth Factor Array
Target
Activin A, BDNF, beta-NGF, bFGF, EGF, Flt-3 Ligand, GDNF, GM-CSF, HGF, IGF-1, PDGF-AA, PDGF-BB, IGFBP-5, VEGF-A
Species Reactivity
Rat
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
A growth factor is a naturally occurring substance capable of stimulating cellular growth, proliferation, healing, and cellular differentiation. Usually it is a protein or a steroid hormone. Growth factors are important for regulating a variety of cellular processes. Growth factors typically act as signaling molecules between cells. Examples are cytokines and hormones that bind to specific receptors on the surface of their target cells. They often promote cell differentiation and maturation, which varies between growth factors. For example, epidermal growth factor (EGF) enhances osteogenic differentiation, while fibroblast growth factors and vascular endothelial growth factors stimulate blood vessel differentiation (angiogenesis).
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Alternative Versions

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Antibody Array

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Related Products

Mouse Growth Factor Antibody Array (Membrane, 20 Targets) (Y0190)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0039)

Human Growth Factor Antibody Array (Glass Slide, 41 Targets) (Y0052)

Human Growth Factor Antibody Array (Membrane, 41 Targets) (Y0256)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0097)

Mouse Growth Factor Antibody Array (Membrane, 30 Targets) (Y0194)

Mouse Growth Factor Antibody Array (Membrane, 10 Targets) (Y0189)

Human Growth Factor Antibody Array (Glass Slide, 40 Targets) (Y0539)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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