Human CD2AP ELISA Kit (V2LY-0626-LY4384)

Name: Human CD2AP ELISA Kit
SKU: V2LY-0626-LY4384
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0055 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

71.5 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CD2 associated protein

Function

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton (PubMed:10339567).
In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation (By similarity).
May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell (By similarity).
May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis (PubMed:15800069).
Plays a role in epithelial cell junctions formation (PubMed:22891260).

Biological Process

Actin filament organization Source: UniProtKB
Cell-cell adhesion Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Negative regulation of small GTPase mediated signal transduction Source: UniProtKB
Negative regulation of transforming growth factor beta1 production Source: Ensembl
Positive regulation of protein localization to nucleus Source: Ensembl
Positive regulation of protein secretion Source: UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
Protein-containing complex assembly Source: ProtInc
Regulation of actin cytoskeleton reorganization Source: Ensembl
Regulation of receptor-mediated endocytosis Source: Ensembl
Signal transduction Source: ProtInc
Substrate-dependent cell migration, cell extension Source: ProtInc
Vesicle organization Source: Ensembl

Cellular Location

Cytoskeleton; Ruffle; Cell junction. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (PubMed:10339567). Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (PubMed:15800069).

Involvement in disease

Focal segmental glomerulosclerosis 3 (FSGS3): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

PTM

Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.

Furusawa, K., Takasugi, T., Chiu, Y. W., Hori, Y., Tomita, T., Fukuda, M., & Hisanaga, S. I. (2019). CD2-associated protein (CD2AP) overexpression accelerates amyloid precursor protein (APP) transfer from early endosomes to the lysosomal degradation pathway. Journal of Biological Chemistry, 294(28), 10886-10899.

Tao, Q. Q., Chen, Y. C., & Wu, Z. Y. (2019). The role of CD2AP in the Pathogenesis of Alzheimer's Disease. Aging and disease, 10(4), 901.

Ha, T. S., & Ha, D. S. (2019). Ginseng total saponin attenuates podocyte apoptosis induced by diabetic conditions through the recovery of CD2-associated protein. Journal of medicinal food, 22(2), 170-177.

Tossidou, I., Teng, B., Worthmann, K., Müller-Deile, J., Jobst-Schwan, T., Kardinal, C., ... & Schiffer, M. (2019). Tyrosine phosphorylation of CD2AP affects stability of the slit diaphragm complex. Journal of the American Society of Nephrology, 30(7), 1220-1237.

Ojelade, S. A., Lee, T. V., Giagtzoglou, N., Yu, L., Ugur, B., Li, Y., ... & Shulman, J. M. (2019). cindr, the Drosophila homolog of the CD2AP Alzheimer’s disease risk gene, is required for synaptic transmission and proteostasis. Cell reports, 28(7), 1799-1813.

Takano, T., Bareke, E., Takeda, N., Aoudjit, L., Baldwin, C., Pisano, P., ... & Gupta, I. R. (2019). Recessive mutation in CD2AP causes focal segmental glomerulosclerosis in humans and mice. Kidney international, 95(1), 57-61.

Zhang, H., Zhang, C., Tang, H., Gao, S., Sun, F., Yang, Y., ... & Li, C. (2018). CD2‐Associated Protein Contributes to Hepatitis C, Virus Propagation and Steatosis by Disrupting Insulin Signaling. Hepatology, 68(5), 1710-1725.

Panni, S. (2018). CD2AP (CD2 associated protein). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Tsuji, K., Păunescu, T. G., Suleiman, H., Xie, D., Mamuya, F. A., Miner, J. H., & Lu, H. A. J. (2017). Re-characterization of the glomerulopathy in CD2AP deficient mice by high-resolution helium ion scanning microscopy. Scientific reports, 7(1), 1-13.

Saurus, P., Tolvanen, T. A., Lindfors, S., Kuusela, S., Holthöfer, H., Lehtonen, E., & Lehtonen, S. (2017). Inhibition of SHIP2 in CD2AP-deficient podocytes ameliorates reactive oxygen species generation but aggravates apoptosis. Scientific reports, 7(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Cd2ap ELISA Kit (CAT#: V2LY-0626-LY1898)

Related Products

Mouse Anti-CD2AP Recombinant Antibody (BR083) (CAT#: CBMAB-BR083LY)

Mouse Cd2ap ELISA Kit (CAT#: V2LY-0626-LY1898)

Isotype control

For research use only. Not intended for any clinical use.

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