Human CD69 ELISA Kit (V2LY-0626-LY4525)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.017 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
22.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD69 Molecule
Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Cellular Location
Membrane
Topology
Cytoplasmic: 1-40
Helical: 41-61
Extracellular: 62-199
PTM
Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Gorabi, A. M., Hajighasemi, S., Kiaie, N., Hayat, S. M. G., Jamialahmadi, T., Johnston, T. P., & Sahebkar, A. (2020). The pivotal role of CD69 in autoimmunity. Journal of autoimmunity, 111, 102453.

Notario, L., Redondo-Antón, J., Alari-Pahissa, E., Albentosa, A., Leiva, M., Lopez, D., ... & Lauzurica, P. (2019). CD69 targeting enhances anti-vaccinia virus immunity. Journal of virology, 93(19), e00553-19.

Kimura, M. Y., Koyama-Nasu, R., Yagi, R., & Nakayama, T. (2019, May). A new therapeutic target: the CD69-Myl9 system in immune responses. In Seminars in immunopathology (Vol. 41, No. 3).

Walsh, D. A., Da Silva, H. B., Beura, L. K., Peng, C., Hamilton, S. E., Masopust, D., & Jameson, S. C. (2019). The functional requirement for CD69 in establishment of resident memory CD8+ T cells varies with tissue location. The Journal of Immunology, 203(4), 946-955.

Brait, V. H., Miró-Mur, F., Pérez-de-Puig, I., Notario, L., Hurtado, B., Pedragosa, J., ... & Planas, A. M. (2019). CD69 plays a beneficial role in ischemic stroke by dampening endothelial activation. Circulation research, 124(2), 279-291.

Mita, Y., Kimura, M. Y., Hayashizaki, K., Koyama-Nasu, R., Ito, T., Motohashi, S., ... & Nakayama, T. (2018). Crucial role of CD69 in anti-tumor immunity through regulating the exhaustion of tumor-infiltrating T cells. International immunology, 30(12), 559-567.

Kimura, M. Y., Igi, A., Hayashizaki, K., Mita, Y., Shinzawa, M., Kadakia, T., ... & Nakayama, T. (2018). CD69 prevents PLZF hi innate precursors from prematurely exiting the thymus and aborting NKT2 cell differentiation. Nature communications, 9(1), 1-12.

Cibrián, D., & Sánchez‐Madrid, F. (2017). CD69: from activation marker to metabolic gatekeeper. European journal of immunology, 47(6), 946-953.

Yong, Y. K., Tan, H. Y., Saeidi, A., Rosmawati, M., Atiya, N., Ansari, A. W., ... & Shankar, E. M. (2017). Decrease of CD69 levels on TCR Vα7. 2+ CD4+ innate-like lymphocytes is associated with impaired cytotoxic functions in chronic hepatitis B virus-infected patients. Innate immunity, 23(5), 459-467.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD69 protein, His Tag (CAT#: V2LY-0526-LY2920)

Human Recombinant CD69 protein, His Tag-1 (CAT#: V2LY-0526-LY2921)

Mouse Recombinant CD69 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8057)

Mouse Recombinant CD69 protein, ECD, His Tag (CAT#: V2LY-0526-LY8058)

CD69 Matched Antibody Pair (233) (CAT#: APMAB-233LY)

Mouse Cd69 ELISA Kit (CAT#: V2LY-0626-LY5866)

Antibody Pairs

CD69 Matched Antibody Pair (233) (CAT#: APMAB-233LY)

Related Products

Hamster Anti-CD69 Recombinant Antibody (CBFYC-0173) (CAT#: CBMAB-C0196-FY)

Mouse Cd69 ELISA Kit (CAT#: V2LY-0626-LY5866)

Mouse Recombinant CD69 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8057)

Mouse Anti-CD69 Recombinant Antibody (CBT314) (CAT#: V2LY-0625-LY3607)

Mouse Anti-CD69 Recombinant Antibody (CBT4039) (CAT#: V2LY-0625-LY2414)

CD69 Matched Antibody Pair (233) (CAT#: APMAB-233LY)

Mouse Anti-CD69 Recombinant Antibody (CBXC-1069) (CAT#: CBMAB-C1333-CQ)

Mouse Anti-CD69 Recombinant Antibody (CBYY-C0635) (CAT#: CBMAB-C1924-YY)

Mouse Anti-CD69 Recombinant Antibody (CBYY-C1555) (CAT#: CBMAB-C2993-YY)

Rabbit Anti-CD69 Recombinant Antibody (006) (CAT#: CBMAB-C1533-LY)

Isotype control

For research use only. Not intended for any clinical use.

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