Human Recombinant CD69 protein, His Tag (V2LY-0526-LY2920)

Name: Human Recombinant CD69 protein, His Tag
SKU: V2LY-0526-LY2920
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CD69 protein, His Tag consist of Amino Acid: 62-199 and predicts a molecular mass of 17.4 kDa.

Molecule Mass

17.4 kDa

Sequence

Amino Acid: 62-199

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD69 Molecule

Function

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Cellular Location

Membrane

Topology

Cytoplasmic: 1-40
Helical: 41-61
Extracellular: 62-199

PTM

Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Gorabi, A. M., Hajighasemi, S., Kiaie, N., Hayat, S. M. G., Jamialahmadi, T., Johnston, T. P., & Sahebkar, A. (2020). The pivotal role of CD69 in autoimmunity. Journal of autoimmunity, 111, 102453.

Notario, L., Redondo-Antón, J., Alari-Pahissa, E., Albentosa, A., Leiva, M., Lopez, D., ... & Lauzurica, P. (2019). CD69 targeting enhances anti-vaccinia virus immunity. Journal of virology, 93(19), e00553-19.

Kimura, M. Y., Koyama-Nasu, R., Yagi, R., & Nakayama, T. (2019, May). A new therapeutic target: the CD69-Myl9 system in immune responses. In Seminars in immunopathology (Vol. 41, No. 3).

Walsh, D. A., Da Silva, H. B., Beura, L. K., Peng, C., Hamilton, S. E., Masopust, D., & Jameson, S. C. (2019). The functional requirement for CD69 in establishment of resident memory CD8+ T cells varies with tissue location. The Journal of Immunology, 203(4), 946-955.

Brait, V. H., Miró-Mur, F., Pérez-de-Puig, I., Notario, L., Hurtado, B., Pedragosa, J., ... & Planas, A. M. (2019). CD69 plays a beneficial role in ischemic stroke by dampening endothelial activation. Circulation research, 124(2), 279-291.

Mita, Y., Kimura, M. Y., Hayashizaki, K., Koyama-Nasu, R., Ito, T., Motohashi, S., ... & Nakayama, T. (2018). Crucial role of CD69 in anti-tumor immunity through regulating the exhaustion of tumor-infiltrating T cells. International immunology, 30(12), 559-567.

Kimura, M. Y., Igi, A., Hayashizaki, K., Mita, Y., Shinzawa, M., Kadakia, T., ... & Nakayama, T. (2018). CD69 prevents PLZF hi innate precursors from prematurely exiting the thymus and aborting NKT2 cell differentiation. Nature communications, 9(1), 1-12.

Cibrián, D., & Sánchez‐Madrid, F. (2017). CD69: from activation marker to metabolic gatekeeper. European journal of immunology, 47(6), 946-953.

Yong, Y. K., Tan, H. Y., Saeidi, A., Rosmawati, M., Atiya, N., Ansari, A. W., ... & Shankar, E. M. (2017). Decrease of CD69 levels on TCR Vα7. 2+ CD4+ innate-like lymphocytes is associated with impaired cytotoxic functions in chronic hepatitis B virus-infected patients. Innate immunity, 23(5), 459-467.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD69 protein, His Tag-1 (CAT#: V2LY-0526-LY2921)

Mouse Recombinant CD69 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8057)

Mouse Recombinant CD69 protein, ECD, His Tag (CAT#: V2LY-0526-LY8058)

CD69 Matched Antibody Pair (233) (CAT#: APMAB-233LY)

Antibody Pairs

CD69 Matched Antibody Pair (233) (CAT#: APMAB-233LY)

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Rabbit Anti-CD69 Recombinant Antibody (006) (CAT#: CBMAB-C1533-LY)

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Mouse Anti-CD69 Recombinant Antibody (CBXC-1069) (CAT#: CBMAB-C1333-CQ)

Mouse Recombinant CD69 protein, ECD, His Tag (CAT#: V2LY-0526-LY8058)

Mouse Anti-CD69 Recombinant Antibody (CBFYC-0311) (CAT#: CBMAB-C0346-FY)

Mouse Recombinant CD69 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8057)

Mouse Anti-CD69 Recombinant Antibody (CBXC-2431) (CAT#: CBMAB-C0039-CQ)

Mouse Anti-CD69 Recombinant Antibody (CBFYC-0508) (CAT#: CBMAB-C0559-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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