Human CHST5 ELISA Kit (V2LY-0626-LY3501)

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Basic Information

Sensitivity
0.048 ng/mL
Detection Range
0.1-35 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
46.2 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5
Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location.
Biological Process
Carbohydrate metabolic process Source: UniProtKB-KW
Keratan sulfate biosynthetic process Source: GO_Central
N-acetylglucosamine metabolic process Source: UniProtKB
Protein sulfation Source: ProtInc
Sulfur compound metabolic process Source: UniProtKB
Cellular Location
Golgi apparatus membrane. Golgi membrane, early secretory pathway.
Topology
Cytoplasmic: 1-30
Helical: 31-48
Lumenal: 49-411
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CHST5 protein, hFc Tag (CAT#: V2LY-0526-LY8113)

Related Products

Mouse Recombinant CHST5 protein, hFc Tag (CAT#: V2LY-0526-LY8113)

Mouse Anti-CHST5 Recombinant Antibody (2E6) (CAT#: CBMAB-C5016-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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