Human CLDN8 ELISA Kit (V2LY-0626-LY2786)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.018 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
24.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Claudin 8
Function
Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cellular Location
Cell membrane; Tight junction. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-117
Helical: 118-138
Extracellular: 139-166
Helical: 167-187
Cytoplasmic: 188-225
PTM
Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.

Zhang, Y., Zheng, A., Lu, H., Jin, Z., Peng, Z., & Jin, F. (2020). The expression and prognostic significance of claudin-8 and androgen receptor in breast cancer. OncoTargets and therapy, 13, 3437.

Li, M., Zhao, J., Cao, M., Liu, R., Chen, G., Li, S., ... & Gong, S. (2020). Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells. Biological research, 53(1), 1-11.

Nattramilarasu, P. K., Bücker, R., Lobo de Sá, F. D., Fromm, A., Nagel, O., Lee, I. F. M., ... & Schulzke, J. D. (2020). Campylobacter concisus impairs sodium absorption in colonic epithelium via ENaC dysfunction and claudin-8 disruption. International journal of molecular sciences, 21(2), 373.

Zhou, S., Piao, X., Wang, C., Wang, R., & Song, Z. (2019). Identification of claudin‑1,‑3,‑7 and‑8 as prognostic markers in human laryngeal carcinoma. Molecular medicine reports, 20(1), 393-400.

Cheng, B., Rong, A., Zhou, Q., & Li, W. (2019). CLDN8 promotes colorectal cancer cell proliferation, migration, and invasion by activating MAPK/ERK signaling. Cancer management and research, 11, 3741.

Li, L., Huang, S., Wang, H., Chao, K., Ding, L., Feng, R., ... & Zhang, S. (2018). Cytokine IL9 triggers the pathogenesis of inflammatory bowel disease through the miR21-CLDN8 pathway. Inflammatory bowel diseases, 24(10), 2211-2223.

Nedjat-Shokouhi, B. (2018). Pathogenesis of Ulcerative Colitis: the role of Claudin-8 in epithelial barrier function and inflammation (Doctoral dissertation, UCL (University College London)).

Ashikari, D., Takayama, K. I., Obinata, D., Takahashi, S., & Inoue, S. (2017). CLDN 8, an androgen‐regulated gene, promotes prostate cancer cell proliferation and migration. Cancer science, 108(7), 1386-1393.

Qi, J., Yang, Y., Hao, P., & Xu, J. (2017). Transcription Factor SOX9 Promotes Osteosarcoma Cell Growth by Repressing Claudin-8 Expression [Retraction]. The Tohoku journal of experimental medicine, 241(1), 55-63.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-CLDN8 Recombinant Antibody (CBXC-1208) (CAT#: V2LY-1206-LY65)

Rabbit Anti-CLDN8 Recombinant Antibody (EG726) (CAT#: V2LY-1206-LY66)

Mouse Anti-CLDN8 Recombinant Antibody (C1337) (CAT#: V2LY-1206-LY64)

Isotype control

For research use only. Not intended for any clinical use.

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