Human CPA3 ELISA Kit (V2LY-0626-LY4860)

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Tested Data
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Basic Information

Sensitivity
0.055 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
48.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
carboxypeptidase A3 (mast cell)
Biological Process
Angiotensin maturation Source: Reactome
Proteolysis Source: GO_Central
Cellular Location
Secretory vesicle. Secretory granules.

Akula, S., Hellman, L., Avilés, F. X., & Wernersson, S. (2022). Analysis of the mast cell expressed carboxypeptidase A3 and its structural and evolutionary relationship to other vertebrate carboxypeptidases. Developmental & Comparative Immunology, 127, 104273.

Atiakshin, D., Kostin, A., Trotsenko, I., Samoilova, V., Buchwalow, I., & Tiemann, M. (2022). Carboxypeptidase A3—A Key Component of the Protease Phenotype of Mast Cells. Cells, 11(3), 570.

Siddhuraj, P., Clausson, C. M., Sanden, C., Alyamani, M., Kadivar, M., Marsal, J., ... & Erjefält, J. S. (2021). Lung mast cells have a high constitutive expression of carboxypeptidase A3 mRNA that is independent from granule-stored CPA3. Cells, 10(2), 309.

Soria‐Castro, R., Meneses‐Preza, Y. G., Rodríguez‐López, G. M., Romero‐Ramírez, S., Sosa‐Hernández, V. A., Cervantes‐Díaz, R., ... & Chacón‐Salinas, R. (2021). Severe COVID‐19 is marked by dysregulated serum levels of carboxypeptidase A3 and serotonin. Journal of leukocyte biology, 110(3), 425-431.

Hämäläinen, S., Kareinen, L., Sukura, A., & Kareinen, I. (2021). Carboxypeptidase A3 expression in canine mast cell tumors and tissue-resident mast cells. Veterinary pathology, 03009858211062636.

Magnúsdóttir, E. I., Grujic, M., Bergman, J., Pejler, G., & Lagerström, M. C. (2020). Mouse connective tissue mast cell proteases tryptase and carboxypeptidase A3 play protective roles in itch induced by endothelin-1. Journal of neuroinflammation, 17(1), 1-11.

Lewicki, Ł., Siebert, J., Koliński, T., Piekarska, K., Reiwer-Gostomska, M., Targoński, R., ... & Marek-Trzonkowska, N. (2019). Mast cell derived carboxypeptidase A3 is decreased among patients with advanced coronary artery disease. Cardiology Journal, 26(6), 680-686.

Abadalkareem, R., Lau, L. C., Abdelmotelb, A., Zhou, X., Eren, E., & Walls, A. F. (2017). Mast cell tryptase and carboxypeptidase A3 (CPA3) as markers for predicting susceptibility to severe allergic drug reactions. Journal of Allergy and Clinical Immunology, 139(2), AB39.

Grujic, M., Paivandy, A., Gustafson, A. M., Thomsen, A. R., Öhrvik, H., & Pejler, G. (2017). The combined action of mast cell chymase, tryptase and carboxypeptidase A3 protects against melanoma colonization of the lung. Oncotarget, 8(15), 25066.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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