Human CS ELISA Kit (V2LY-0626-LY2106)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
51.7 kDa
Components
  • Standard Solution: 0.5ml x1
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard Diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop Solution: 6ml x1
  • Substrate Solution A: 6ml x1
  • Substrate Solution B: 6ml x1
  • Wash Buffer Concentrate (25x): 20ml x1
  • Biotinylated Antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Citrate Synthase
Biological Process
Carbohydrate metabolic process Source: UniProtKB
Citrate metabolic process Source: InterPro
Tricarboxylic acid cycle Source: GO_Central
Cellular Location
Mitochondrion matrix
PTM
Methylated (PubMed:28391595, PubMed:28887308). Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity (PubMed:28887308).

Zhao, H., Chen, G., Sang, L., Deng, Y., Gao, L., Yu, Y., & Liu, J. (2021). Mitochondrial citrate synthase plays important roles in anthocyanin synthesis in petunia. Plant Science, 305, 110835.

Cai, Z., Deng, Y., Ye, J., Zhuo, Y., Liu, Z., Liang, Y., ... & Zhong, W. (2020). Aberrant expression of citrate synthase is linked to disease progression and clinical outcome in prostate cancer. Cancer Management and Research, 12, 6149.

Ito, S., Koyama, N., & Osanai, T. (2019). Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase. Scientific reports, 9(1), 1-9.

Verschueren, K. H., Blanchet, C., Felix, J., Dansercoer, A., De Vos, D., Bloch, Y., ... & Verstraete, K. (2019). Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature, 568(7753), 571-575.

Alhindi, Y., Vaanholt, L. M., Al-Tarrah, M., Gray, S. R., Speakman, J. R., Hambly, C., ... & Ratkevicius, A. (2019). Low citrate synthase activity is associated with glucose intolerance and lipotoxicity. Journal of nutrition and metabolism, 2019.

Mustafa, G., Arif, R., Bukhari, S. A., Ali, M., Sharif, S., & Atta, A. (2018). Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120. Pakistan journal of pharmaceutical sciences, 31.

Rhein, V. F., Carroll, J., Ding, S., Fearnley, I. M., & Walker, J. E. (2017). Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS letters, 591(12), 1641-1652.

Cui, X. X., Li, X., Dong, S. Y., Guo, Y. J., Liu, T., & Wu, Y. C. (2017). SIRT3 deacetylated and increased citrate synthase activity in PD model. Biochemical and biophysical research communications, 484(4), 767-773.

Cai, Q., Zhao, M., Liu, X., Wang, X., Nie, Y., Li, P., ... & Han, F. (2017). Reduced expression of citrate synthase leads to excessive superoxide formation and cell apoptosis. Biochemical and biophysical research communications, 485(2), 388-394.

MacPherson, S., Horkoff, M., Gravel, C., Hoffmann, T., Zuber, J., & Lum, J. J. (2017). STAT3 regulation of citrate synthase is essential during the initiation of lymphocyte cell growth. Cell reports, 19(5), 910-918.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CS protein, His Tag (CAT#: V2LY-0526-LY3143)

Human CS ELISA Kit (2) (CAT#: V2LY-0626-LY4413)

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Isotype control

For research use only. Not intended for any clinical use.

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