Human Recombinant CS protein, His Tag (V2LY-0526-LY3143)

Name: Human Recombinant CS protein, His Tag
SKU: V2LY-0526-LY3143
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CS protein, His Tag consist of Amino Acid: 1-466 and predicts a molecular mass of 50.4 kDa.

Molecule Mass

50.4 kDa

Sequence

Amino Acid: 1-466

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Citrate Synthase

Biological Process

Carbohydrate metabolic process Source: UniProtKB
Citrate metabolic process Source: InterPro
Tricarboxylic acid cycle Source: GO_Central

Cellular Location

Mitochondrion matrix

PTM

Methylated (PubMed:28391595, PubMed:28887308). Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity (PubMed:28887308).

Zhao, H., Chen, G., Sang, L., Deng, Y., Gao, L., Yu, Y., & Liu, J. (2021). Mitochondrial citrate synthase plays important roles in anthocyanin synthesis in petunia. Plant Science, 305, 110835.

Cai, Z., Deng, Y., Ye, J., Zhuo, Y., Liu, Z., Liang, Y., ... & Zhong, W. (2020). Aberrant expression of citrate synthase is linked to disease progression and clinical outcome in prostate cancer. Cancer Management and Research, 12, 6149.

Ito, S., Koyama, N., & Osanai, T. (2019). Citrate synthase from Synechocystis is a distinct class of bacterial citrate synthase. Scientific reports, 9(1), 1-9.

Verschueren, K. H., Blanchet, C., Felix, J., Dansercoer, A., De Vos, D., Bloch, Y., ... & Verstraete, K. (2019). Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature, 568(7753), 571-575.

Alhindi, Y., Vaanholt, L. M., Al-Tarrah, M., Gray, S. R., Speakman, J. R., Hambly, C., ... & Ratkevicius, A. (2019). Low citrate synthase activity is associated with glucose intolerance and lipotoxicity. Journal of nutrition and metabolism, 2019.

Mustafa, G., Arif, R., Bukhari, S. A., Ali, M., Sharif, S., & Atta, A. (2018). Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120. Pakistan journal of pharmaceutical sciences, 31.

Rhein, V. F., Carroll, J., Ding, S., Fearnley, I. M., & Walker, J. E. (2017). Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase. FEBS letters, 591(12), 1641-1652.

Cui, X. X., Li, X., Dong, S. Y., Guo, Y. J., Liu, T., & Wu, Y. C. (2017). SIRT3 deacetylated and increased citrate synthase activity in PD model. Biochemical and biophysical research communications, 484(4), 767-773.

Cai, Q., Zhao, M., Liu, X., Wang, X., Nie, Y., Li, P., ... & Han, F. (2017). Reduced expression of citrate synthase leads to excessive superoxide formation and cell apoptosis. Biochemical and biophysical research communications, 485(2), 388-394.

MacPherson, S., Horkoff, M., Gravel, C., Hoffmann, T., Zuber, J., & Lum, J. J. (2017). STAT3 regulation of citrate synthase is essential during the initiation of lymphocyte cell growth. Cell reports, 19(5), 910-918.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human CS ELISA Kit (CAT#: V2LY-0626-LY2106)

Human CS ELISA Kit (2) (CAT#: V2LY-0626-LY4413)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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