Human CYGB ELISA Kit (V2LY-0626-LY2356)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
21.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cytoglobin
Function
May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.
Biological Process
Oxygen transport Source: Reactome
Regulation of nitric-oxide synthase activity Source: Reactome
Response to oxidative stress Source: UniProtKB
Cellular Location
Cytoplasm

Zweier, J. L., Hemann, C., Kundu, T., Ewees, M. G., Khaleel, S. A., Samouilov, A., ... & El-Mahdy, M. A. (2021). Cytoglobin has potent superoxide dismutase function. Proceedings of the National Academy of Sciences, 118(52).

Dat, N. Q., Thuy, L. T. T., Hieu, V. N., Hai, H., Hoang, D. V., Thi Thanh Hai, N., ... & Kawada, N. (2021). Hexa Histidine–Tagged Recombinant Human Cytoglobin Deactivates Hepatic Stellate Cells and Inhibits Liver Fibrosis by Scavenging Reactive Oxygen Species. Hepatology, 73(6), 2527-2545.

De Backer, J., Maric, D., Bosman, M., Dewilde, S., & Hoogewijs, D. (2021). A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma. Scientific reports, 11(1), 1-11.

Zweier, J. L., & Ilangovan, G. (2020). Regulation of nitric oxide metabolism and vascular tone by cytoglobin. Antioxidants & Redox Signaling, 32(16), 1172-1187.

Le Thi Thanh Thuy, H. H., & Kawada, N. (2020). Role of cytoglobin, a novel radical scavenger, in stellate cell activation and hepatic fibrosis. Clinical and molecular hepatology, 26(3), 280.

Okina, Y., Sato-Matsubara, M., Matsubara, T., Daikoku, A., Longato, L., Rombouts, K., ... & Kawada, N. (2020). TGF-β1-driven reduction of cytoglobin leads to oxidative DNA damage in stellate cells during non-alcoholic steatohepatitis. Journal of hepatology, 73(4), 882-895.

De Backer, J., Razzokov, J., Hammerschmid, D., Mensch, C., Hafideddine, Z., Kumar, N., ... & Dewilde, S. (2018). The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor. Redox biology, 19, 1-10.

Jourd’heuil, F. L., Xu, H., Reilly, T., McKellar, K., El Alaoui, C., Steppich, J., ... & Jourd’heuil, D. (2017). The hemoglobin homolog cytoglobin in smooth muscle inhibits apoptosis and regulates vascular remodeling. Arteriosclerosis, thrombosis, and vascular biology, 37(10), 1944-1955.

Xu, Z. J., Shu, S., Li, Z. J., Liu, Y. M., Zhang, R. Y., & Zhang, Y. (2017). Liuwei Dihuang pill treats diabetic nephropathy in rats by inhibiting of TGF-β/SMADS, MAPK, and NF-kB and upregulating expression of cytoglobin in renal tissues. Medicine, 96(3).

Liu, X., El-Mahdy, M. A., Boslett, J., Varadharaj, S., Hemann, C., Abdelghany, T. M., ... & Zweier, J. L. (2017). Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall. Nature communications, 8(1), 1-14.

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For research use only. Not intended for any clinical use.

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