Human CYP26A1 ELISA Kit (V2LY-0626-LY4473)

Name: Human CYP26A1 ELISA Kit
SKU: V2LY-0626-LY4473
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0049 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

56.2 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Cytochrome P450 Family 26 Subfamily A Member 1

Function

A cytochrome P450 monooxygenase involved in the metabolism of all-trans retinoic acid (atRA), a signaling molecule that binds to retinoic acid receptors and regulates gene transcription. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4 and C-18. Has no activity toward 9-cis and 13-cis retinoic acid stereoisomers (PubMed:22020119, PubMed:9228017, PubMed:9716180).

May play a role in the oxidative metabolism of xenobiotics such as tazarotenic acid (PubMed:26937021).

Biological Process

Kidney development Source: Ensembl
Negative regulation of retinoic acid receptor signaling pathway Source: BHF-UCL
Response to retinoic acid Source: Ensembl
Response to vitamin A Source: Ensembl
Retinoic acid catabolic process Source: BHF-UCL
Retinoic acid metabolic process Source: UniProtKB
Sterol metabolic process Source: GO_Central
Vitamin metabolic process Source: Reactome
Xenobiotic metabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Horiguchi, K., Fujiwara, K., Tsukada, T., Nakakura, T., Yoshida, S., Hasegawa, R., & Takigami, S. (2022). The multiciliated cells in Rathke’s cleft express CYP26A1 and respond to retinoic acid in the pituitary. Cell and Tissue Research, 1-12.

Chen, P. H., Chung, C. M., Wang, Y. Y., Huang, H. W., Huang, B., Lee, K. W., ... & Chan, L. P. (2020). CYP26A1 is a novel biomarker for betel quid-related oral and pharyngeal cancers. Diagnostics, 10(11), 982.

Sharma, S., Liu, J., Zhang, X., Sharma, S. S., Sorensen, E. J., & Bureik, M. (2020). New luciferin-based probe substrates for human CYP26A1. Biochemistry and biophysics reports, 24, 100861.

Snyder, J. M., Zhong, G., Hogarth, C., Huang, W., Topping, T., LaFrance, J., ... & Isoherranen, N. (2020). Knockout of Cyp26a1 and Cyp26b1 during postnatal life causes reduced lifespan, dermatitis, splenomegaly, and systemic inflammation in mice. The FASEB Journal, 34(12), 15788-15804.

Guo, P., Ji, Z., Jiang, H., Huang, X., Wang, C., & Pan, B. (2020). Identification of a novel CYP26A1 mutation in a Chinese family with congenital microtia. International Journal of Pediatric Otorhinolaryngology, 139, 110488.

Zhong, G., Hogarth, C., Snyder, J. M., Palau, L., Topping, T., Huang, W., ... & Isoherranen, N. (2019). The retinoic acid hydroxylase Cyp26a1 has minor effects on postnatal vitamin A homeostasis, but is required for exogenous atRA clearance. Journal of Biological Chemistry, 294(29), 11166-11179.

Zolfaghari, R., Mattie, F. J., Wei, C. H., Chisholm, D. R., Whiting, A., & Ross, A. C. (2019). CYP26A1 gene promoter is a useful tool for reporting RAR-mediated retinoid activity. Analytical biochemistry, 577, 98-109.

Jin, L., Chang, C., Pawlik, K. M., Datta, A., Johnson, L. M., Vu, T., ... & Datta, P. K. (2018). Serine threonine kinase receptor-associated protein deficiency impairs mouse embryonic stem cells lineage commitment through CYP26A1-mediated retinoic acid homeostasis. Stem Cells, 36(9), 1368-1379.

Stevison, F., Hogarth, C., Tripathy, S., Kent, T., & Isoherranen, N. (2017). Inhibition of the all-trans retinoic acid (atRA) hydroxylases CYP26A1 and CYP26B1 results in dynamic, tissue-specific changes in endogenous atRA signaling. Drug Metabolism and Disposition, 45(7), 846-854.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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