Human CYP2C9 ELISA Kit (V2LY-0626-LY4474)

Name: Human CYP2C9 ELISA Kit
SKU: V2LY-0626-LY4474
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0076 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

55.6 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Cytochrome P450 Family 2 Subfamily C Member 9

Function

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599).

Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed:7574697, PubMed:15766564, PubMed:19965576, PubMed:9866708).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599).

Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed:12865317).

Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA) (PubMed:9866708, PubMed:9435160).

Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794).

Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed:25994031).

Biological Process

Cellular amide metabolic process Source: BHF-UCL
Cholesterol metabolic process Source: UniProtKB-UniPathway
Drug catabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: UniProtKB
Estrogen metabolic process Source: UniProtKB
Exogenous drug catabolic process Source: BHF-UCL
Icosanoid biosynthetic process Source: UniProtKB
Long-chain fatty acid biosynthetic process Source: Reactome
Monocarboxylic acid metabolic process Source: BHF-UCL
Monoterpenoid metabolic process Source: BHF-UCL
Omega-hydroxylase P450 pathway Source: Reactome
Organic acid metabolic process Source: GO_Central
Oxidative demethylation Source: BHF-UCL
Steroid metabolic process Source: BHF-UCL
Urea metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Sangkuhl, K., Claudio‐Campos, K., Cavallari, L. H., Agundez, J. A., Whirl‐Carrillo, M., Duconge, J., ... & Gaedigk, A. (2021). PharmVar GeneFocus: CYP2C9. Clinical Pharmacology & Therapeutics, 110(3), 662-676.

Amorosi, C. J., Chiasson, M. A., McDonald, M. G., Wong, L. H., Sitko, K. A., Boyle, G., ... & Dunham, M. J. (2021). Massively parallel characterization of CYP2C9 variant enzyme activity and abundance. The American Journal of Human Genetics, 108(9), 1735-1751.

Chen, H., Dai, D. P., Zhou, S., Liu, J., Wang, S. H., Wu, H. L., ... & Yang, J. F. (2020). An identification and functional evaluation of a novel CYP2C9 variant CYP2C9* 62. Chemico-Biological Interactions, 327, 109168.

Theken, K. N., Lee, C. R., Gong, L., Caudle, K. E., Formea, C. M., Gaedigk, A., ... & Grosser, T. (2020). Clinical Pharmacogenetics Implementation Consortium Guideline (CPIC) for CYP2C9 and nonsteroidal anti‐inflammatory drugs. Clinical Pharmacology & Therapeutics, 108(2), 191-200.

Monostory, K., Nagy, A., Tóth, K., Bűdi, T., Kiss, Á., Déri, M., & Csukly, G. (2019). Relevance of CYP2C9 function in valproate therapy. Current neuropharmacology, 17(1), 99-106.

Louet, M., Labbé, C. M., Fagnen, C., Aono, C. M., Homem-de-Mello, P., Villoutreix, B. O., & Miteva, M. A. (2018). Insights into molecular mechanisms of drug metabolism dysfunction of human CYP2C9* 30. PloS one, 13(5), e0197249.

Silvado, C. E., Terra, V. C., & Twardowschy, C. A. (2018). CYP2C9 polymorphisms in epilepsy: influence on phenytoin treatment. Pharmacogenomics and Personalized Medicine, 11, 51.

Flora, D. R., Rettie, A. E., Brundage, R. C., & Tracy, T. S. (2017). CYP2C9 genotype‐dependent warfarin pharmacokinetics: impact of CYP2C9 genotype on R‐and S‐warfarin and their oxidative metabolites. The Journal of Clinical Pharmacology, 57(3), 382-393.

Daly, A. K., Rettie, A. E., Fowler, D. M., & Miners, J. O. (2017). Pharmacogenomics of CYP2C9: functional and clinical considerations. Journal of personalized medicine, 8(1), 1.

Liu, R., Lyu, X., Batt, S. M., Hsu, M. H., Harbut, M. B., Vilchèze, C., ... & Wang, F. (2017). Determinants of the Inhibition of DprE1 and CYP2C9 by Antitubercular Thiophenes. Angewandte Chemie International Edition, 56(42), 13011-13015.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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