Human FGF5 ELISA Kit (V2LY-0626-LY4583)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0053 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
29.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Fibroblast Growth Factor 5
Research Area
Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase (By similarity).
Biological Process
Animal organ morphogenesis Source: GO_Central
Cell-cell signaling Source: ProtInc
Cell differentiation Source: GO_Central
Fibroblast growth factor receptor signaling pathway Source: MGI
Glial cell differentiation Source: Ensembl
Nervous system development Source: ProtInc
Positive regulation of cell division Source: UniProtKB-KW
Positive regulation of cell population proliferation Source: MGI
Positive regulation of gene expression Source: GO_Central
Positive regulation of protein phosphorylation Source: GO_Central
Regulation of cell migration Source: GO_Central
Signal transduction involved in regulation of gene expression Source: Ensembl
Cellular Location
Secreted
Involvement in disease
Trichomegaly (TCMGLY):
A morphologic trait characterized by unusually long eyelashes and mild hypertrichosis of eyebrows. It can be observed in association with corneal irritation, cataracts, and hereditary spherocytosis.

Zhang, G., Zheng, H., & Wang, L. (2022). miR‑491‑3p functions as a tumor suppressor in non‑small cell lung cancer by targeting fibroblast growth factor 5. Oncology Reports, 48(3), 1-12.

Hoang, S. H. (2022). Fibroblast Growth Factor 5 (FGF5) and Its Missense Mutant FGF5-Y174H Underlying Trichomegaly: A Molecular Dynamics Simulation Investigation. bioRxiv.

Shaffer, G. D., Ballif, B. C., Meurs, K., Shaffer, L. G., & Flores-Smith, H. (2021). Identification of a novel missense mutation in the fibroblast growth factor 5 gene associated with longhair in the Maine Coon Cat. Human Genetics, 140(11), 1517-1523.

Xu, Y., Liu, H., Pan, H., Wang, X., Zhang, Y., Yao, B., ... & Li, Z. (2020). CRISPR/Cas9-mediated disruption of fibroblast growth factor 5 in rabbits results in a systemic long hair phenotype by prolonging anagen. Genes, 11(3), 297.

Li, H., & Lipsett, K. A. (2019). The Association of Fibroblast Growth Factor 5 (FGF5), Fibroblast Growth Factor Receptor 1 (FGFR1), and Prolactin (PRL) with Hair Length in Scottish Highland Cattle. The FASEB Journal, 33(S1), 474-9.

McLean*, D., Cunningham, K., & Ricke, W. (2019). Mp68-20 establishing a negative feedback loop between fibroblast growth factor 5 and androgen receptor in prostate cancer. The Journal of Urology, 201(Supplement 4), e983-e983.

Zhou, Y., Yu, Q., Chu, Y., Zhu, X., Deng, J., Liu, Q., & Wang, Q. (2019). Downregulation of fibroblast growth factor 5 inhibits cell growth and invasion of human nonsmall‐cell lung cancer cells. Journal of Cellular Biochemistry, 120(5), 8238-8246.

Huang, Y., Wang, H., & Yang, Y. (2018). Expression of fibroblast growth factor 5 (FGF5) and its influence on survival of breast cancer patients. Medical science monitor: international medical journal of experimental and clinical research, 24, 3524.

Ren, Y., Jiao, X., & Zhang, L. (2018). Expression level of fibroblast growth factor 5 (FGF5) in the peripheral blood of primary hypertension and its clinical significance. Saudi journal of biological sciences, 25(3), 469-473.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Fgf5 ELISA Kit (CAT#: V2LY-0626-LY5865)

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Mouse Anti-FGF5 Recombinant Antibody (CBFYH-0193) (CAT#: V2LY-0925-LY1585)

Mouse Anti-FGF5 Recombinant Antibody (CBXF-0584) (CAT#: V2LY-0925-LY1588)

Isotype control

For research use only. Not intended for any clinical use.

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