Human GPC4 ELISA Kit (V2LY-0626-LY3594)

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Tested Data
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Basic Information

Sensitivity
0.14 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
62.4 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
glypican 4
Function
Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).
Biological Process
Cell migration Source: GO_Central
Regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: GO_Central
Regulation of presynapse assembly Source: GO_Central
Regulation of protein localization to membrane Source: GO_Central
Regulation of signal transduction Source: InterPro
Synaptic membrane adhesion Source: GO_Central
Wnt signaling pathway Source: UniProtKB
Cellular Location
Cell membrane
Secreted glypican-4: Extracellular space
Involvement in disease
Keipert syndrome (KPTS):
An X-linked recessive syndrome characterized by craniofacial and digital abnormalities. Clinical features include a prominent forehead, a flat midface, hypertelorism, a broad nose, downturned corners of mouth, and widening of all distal phalanges. Additional variable features are cognitive impairment and sensorineural deafness.

Saroja, S. R., Gorbachev, K., TCW, J., Goate, A. M., & Pereira, A. C. (2022). Astrocyte-secreted glypican-4 drives APOE4-dependent tau hyperphosphorylation. Proceedings of the National Academy of Sciences, 119(34), e2108870119.

Muendlein, A., Brandtner, E. M., Leiherer, A., Geiger, K., Heinzle, C., Gaenger, S., ... & Drexel, H. (2022). Evaluation of the association of serum glypican-4 with prevalent and future kidney function. Scientific Reports, 12(1), 1-8.

Muendlein, A., Brandtner, E. M., Leiherer, A., Geiger, K., Heinzle, C., Gaenger, S., ... & Drexel, H. (2022). Data on the association of serum glypican-4 with future major adverse cardiovascular events and mortality in patients undergoing coronary angiography. Data in Brief, 42, 108142.

Muendlein, A., Brandtner, E. M., Leiherer, A., Geiger, K., Heinzle, C., Gaenger, S., ... & Drexel, H. (2022). Serum glypican-4 is a marker of future vascular risk and mortality in coronary angiography patients. Atherosclerosis, 345, 33-38.

Hu, B., Rodriguez, J. J., Kakkerla Balaraju, A., Gao, Y., Nguyen, N. T., Steen, H., ... & Lin, F. (2021). Glypican 4 mediates Wnt transport between germ layers via signaling filopodia. Journal of Cell Biology, 220(12), e202009082.

Ma, K., Xing, S., Luan, Y., Zhang, C., Liu, Y., Fei, Y., ... & Chen, X. (2021). Glypican 4 regulates Aβ internalization in neural stem cells partly via low-density lipoprotein receptor-related protein 1. Frontiers in Cellular Neuroscience, 15, 732429.

Munir, J., Van Ngu, T., Ayudthaya, P. D. N., & Ryu, S. (2020). Downregulation of glypican-4 facilitates breast cancer progression by inducing cell migration and proliferation. Biochemical and Biophysical Research Communications, 526(1), 91-97.

Hu, B., Gao, Y., Davies, L., Woo, S., Topczewski, J., Jessen, J. R., & Lin, F. (2018). Glypican 4 and Mmp14 interact in regulating the migration of anterior endodermal cells by limiting extracellular matrix deposition. Development, 145(17), dev163303.

Dowling, C., & Allen, N. J. (2018). Mice lacking glypican 4 display juvenile hyperactivity and adult social interaction deficits. Brain Plasticity, 4(2), 197-209.

Cao, J., Ma, J., Sun, L., Li, J., Qin, T., Zhou, C., ... & Han, L. (2018). Targeting glypican‐4 overcomes 5‐FU resistance and attenuates stem cell–like properties via suppression of Wnt/β‐catenin pathway in pancreatic cancer cells. Journal of cellular biochemistry, 119(11), 9498-9512.

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For research use only. Not intended for any clinical use.

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