Human GRK5 ELISA Kit (2) (V2LY-0626-LY4601)

Name: Human GRK5 ELISA Kit (2)
SKU: V2LY-0626-LY4601
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.01 ng/mL

Detection Range

0.02-3.8 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

67.8 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

G protein-coupled receptor kinase 5

Biological Process

Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: ProtInc
Apoptotic process Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of cell population proliferation Source: CACAO
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: GO_Central
Regulation of cell cycle Source: CACAO
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of signal transduction Source: GO_Central
Tachykinin receptor signaling pathway Source: BHF-UCL
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Cell membrane; Nucleus; Cytoplasm. Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca2+/calmodulin-dependent fashion.

PTM

Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.

De Lucia, C., Grisanti, L. A., Borghetti, G., Piedepalumbo, M., Ibetti, J., Lucchese, A. M., ... & Koch, W. J. (2022). G protein-coupled receptor kinase 5 (GRK5) contributes to impaired cardiac function and immune cell recruitment in post-ischemic heart failure. Cardiovascular Research, 118(1), 169-183.

Downes, K., Zhao, X., Gleadall, N. S., McKinney, H., Kempster, C., Batista, J., ... & Ma, P. (2022). G protein–coupled receptor kinase 5 regulates thrombin signaling in platelets via PAR-1. Blood Advances, 6(7), 2319-2330.

Toya, M., Akasaki, Y., Sueishi, T., Kurakazu, I., Kuwahara, M., Uchida, T., ... & Nakashima, Y. (2021). G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis. Scientific reports, 11(1), 10481.

Guimarães, T. R., Swanson, E., Kofler, J., & Thathiah, A. (2021). G protein‐coupled receptor kinases are associated with Alzheimer's disease pathology. Neuropathology and applied neurobiology, 47(7), 942-957.

Sok, V., Jacinto, A. Z., Peng, N., Eldemerdash, M., Le, L., Tran, P. D., ... & So, C. H. (2021). G protein coupled receptor kinase 5 modifies the nucleolar stress response activated by actinomycin D. Biochemistry and Cell Biology, 99(4), 508-518.

Lagman, J., Sayegh, P., Lee, C. S., Sulon, S. M., Jacinto, A. Z., Sok, V., ... & So, C. H. (2019). G protein-coupled receptor kinase 5 modifies cancer cell resistance to paclitaxel. Molecular and Cellular Biochemistry, 461, 103-118.

Rowlands, R. A., Cato, M. C., Waldschmidt, H. V., Bouley, R. A., Chen, Q., Avramova, L., ... & White, A. D. (2019). Structure-based design of selective, covalent G protein-coupled receptor kinase 5 inhibitors. ACS Medicinal Chemistry Letters, 10(12), 1628-1634.

Yeh, S. T., Zambrano, C. M., Koch, W. J., & Purcell, N. H. (2018). PH domain leucine-rich repeat protein phosphatase 2 (PHLPP2) regulates G-protein–coupled receptor kinase 5 (GRK5)-induced cardiac hypertrophy in vitro. Journal of Biological Chemistry, 293(21), 8056-8064.

Lutz, S. Z., Falcenberg, M., Machicao, F., Peter, A., Kächele, M., Randrianarisoa, E., ... & Kantartzis, K. (2018). Single Nucleotide Polymorphisms in the G-Protein Coupled Receptor Kinase 5 (GRK5) Gene are associated with Plasma LDL-Cholesterol Levels in Humans. Scientific Reports, 8(1), 7745.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GRK5, Active protein, GST Tag (CAT#: V2LY-0526-LY4418)

Human Recombinant GRK5 protein, His Tag (CAT#: V2LY-0526-LY4419)

Human GRK5 ELISA Kit (CAT#: V2LY-0626-LY4270)

Related Products

Mouse Anti-GRK5 Recombinant Antibody (CBFYH-0488) (CAT#: CBMAB-H0276-FY)

Human Recombinant GRK5 protein, His Tag (CAT#: V2LY-0526-LY4419)

Mouse Anti-GRK5 Recombinant Antibody (CBFYH-0489) (CAT#: CBMAB-H1358-FY)

Mouse Anti-GRK5 Monoclonal Antibody (16) (CAT#: CBMAB-G5012-LY)

Human Recombinant GRK5, Active protein, GST Tag (CAT#: V2LY-0526-LY4418)

Mouse Anti-GRK5 Monoclonal Antibody (20) (CAT#: CBMAB-G0806-LY)

Human GRK5 ELISA Kit (CAT#: V2LY-0626-LY4270)

Mouse Anti-GRK5 Monoclonal Antibody (10A12) (CAT#: V2LY-0226-LY1337)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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