Human GZMH ELISA Kit (V2LY-0626-LY4637)

Name: Human GZMH ELISA Kit
SKU: V2LY-0626-LY4637
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0055 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

27.3 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

granzyme H

Function

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.

Biological Process

Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW

Cellular Location

Cytolytic granule

Yu, D., Cai, W., Chen, X., Lu, D., Hu, M., Lu, T., ... & Lu, Z. (2023). Natural Killer Cells Disrupt Nerve Fibers by Granzyme H in Atheriosclerotic Cerebral Small Vessel Disease. The Journals of Gerontology: Series A, 78(3), 414-423.

Ikram, S., Ahmad, F., Ahmad, J., & Durdagi, S. (2021). Screening of small molecule libraries using combined text mining, ligand-and target-driven based approaches for identification of novel granzyme H inhibitors. Journal of Molecular Graphics and Modelling, 105, 107876.

Bongards, E. N. (2021). Linking Granzyme H to NK-Cell Education–An Examination of Expression Patterns and Functions (Doctoral dissertation).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-GZMH Recombinant Antibody (CBFYH-3495) (CAT#: CBMAB-H4045-FY)

Mouse Anti-GZMH Recombinant Antibody (10F4) (CAT#: CBMAB-G5961-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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