Human HCN2 ELISA Kit (V2LY-0626-LY4277)

Name: Human HCN2 ELISA Kit
SKU: V2LY-0626-LY4277
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.01 ng/mL

Detection Range

0.02-4 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

97.0 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2

Function

Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).

Biological Process

Cell-cell signaling Source: ProtInc
Cellular response to cAMP Source: UniProtKB
Cellular response to cGMP Source: UniProtKB
Membrane depolarization during cardiac muscle cell action potential Source: BHF-UCL
Potassium ion import across plasma membrane Source: BHF-UCL
Potassium ion transmembrane transport Source: UniProtKB
Regulation of ion transmembrane transport Source: UniProtKB-KW
Regulation of membrane depolarization Source: ComplexPortal
Regulation of membrane potential Source: UniProtKB
Sodium ion import across plasma membrane Source: BHF-UCL
Sodium ion transmembrane transport Source: UniProtKB

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-215
Helical: 216-236
Extracellular: 237-240
Helical: 241-261
Cytoplasmic: 262-288
Helical: 289-309
Extracellular: 310-317
Helical: 318-338
Cytoplasmic: 339-369
Helical: 370-390
Extracellular: 391-413
Pore-forming: 414-435
Extracellular: 436-440
Helical: 441-461
Cytoplasmic: 462-889

PTM

Phosphorylation at Ser-668 by PRKG2 shifts the voltage-dependence to more negative voltages, hence counteracting the stimulatory effect of cGMP on gating.

Nordström, T., Andersson, L. C., & Åkerman, K. E. (2022). Role of hyperpolarization-activated cyclic nucleotide-gated channel HCN2 in embryonic neural stem cell proliferation and differentiation. Neurochemistry International, 159, 105387.

Forster, L. (2022). Regulation of the hyperpolarization-activated cyclic nucleotide gated ion channel 2, HCN2, by post-translational SUMOylation.

Forster, L. A., Jansen, L. A. R., Rubaharan, M., Murphy, A. Z., & Baro, D. J. (2020). Alterations in SUMOylation of the hyperpolarization‐activated cyclic nucleotide‐gated ion channel 2 during persistent inflammation. European Journal of Pain, 24(8), 1517-1536.

Yuan, L., Luo, L., Ma, X., Wang, W., Yu, K., Shi, H., ... & Xu, T. (2020). Chronic morphine induces cyclic adenosine monophosphate formation and hyperpolarization-activated cyclic nucleotide-gated channel expression in the spinal cord of mice. Neuropharmacology, 176, 108222.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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