Human HIF1AN ELISA Kit (V2LY-0626-LY3619)

Name: Human HIF1AN ELISA Kit
SKU: V2LY-0626-LY3619
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0051 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

40.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor

Function

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Biological Process

Negative regulation of Notch signaling pathway Source: UniProtKB
Negative regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: UniProtKB
Peptidyl-asparagine hydroxylation Source: UniProtKB
Peptidyl-aspartic acid hydroxylation Source: UniProtKB
Peptidyl-histidine hydroxylation Source: UniProtKB
Positive regulation of myoblast differentiation Source: UniProtKB
Positive regulation of vasculogenesis Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm; Perinuclear region. Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.

Kunej, T. (2021). Integrative map of HIF1A regulatory elements and variations. Genes, 12(10), 1526.

He, R., Wang, Z., Cui, M., Liu, S., Wu, W., Chen, M., ... & Shao, Z. (2021). HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy. Autophagy, 17(11), 3338-3360.

Lin, Q., Li, S., Jiang, N., Jin, H., Shao, X., Zhu, X., ... & Ni, Z. (2021). Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy. Autophagy, 17(10), 2975-2990.

Xu, F., Huang, M., Chen, Q., Niu, Y., Hu, Y., Hu, P., ... & Zhao, G. (2021). LncRNA HIF1A-AS1 promotes gemcitabine resistance of pancreatic cancer by enhancing glycolysis through modulating the AKT/YB1/HIF1α pathway. Cancer Research, 81(22), 5678-5691.

Tiwari, A., Tashiro, K., Dixit, A., Soni, A., Vogel, K., Hall, B., ... & Bagchi, A. (2020). Loss of HIF1A from pancreatic cancer cells increases expression of PPP1R1B and degradation of p53 to promote invasion and metastasis. Gastroenterology, 159(5), 1882-1897.

Ou, Z. L., Luo, Z., Wei, W., Liang, S., Gao, T. L., & Lu, Y. B. (2019). Hypoxia-induced shedding of MICA and HIF1A-mediated immune escape of pancreatic cancer cells from NK cells: role of circ_0000977/miR-153 axis. RNA biology, 16(11), 1592-1603.

Yin, N., Zhu, L., Ding, L., Yuan, J., Du, L., Pan, M., ... & Xiao, H. (2019). MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells. Cellular & molecular biology letters, 24, 1-11.

Cimmino, F., Avitabile, M., Lasorsa, V. A., Montella, A., Pezone, L., Cantalupo, S., ... & Capasso, M. (2019). HIF-1 transcription activity: HIF1A driven response in normoxia and in hypoxia. BMC medical genetics, 20, 1-15.

Ebersole, J. L., Novak, M. J., Orraca, L., Martinez‐Gonzalez, J., Kirakodu, S., Chen, K. C., ... & Gonzalez, O. A. (2018). Hypoxia‐inducible transcription factors, HIF1A and HIF2A, increase in aging mucosal tissues. Immunology, 154(3), 452-464.

Wang, R., Ma, Z., Feng, L., Yang, Y., Tan, C., Shi, Q., ... & Fang, J. (2018). LncRNA MIR31HG targets HIF1A and P21 to facilitate head and neck cancer cell proliferation and tumorigenesis by promoting cell-cycle progression. Molecular cancer, 17(1), 1-6.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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