Human HNF1A ELISA Kit (V2LY-0626-LY5611)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0071 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
67.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
HNF1 Homeobox A
Function
Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity).

Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:12453420, PubMed:10966642).

Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).
Biological Process
Glucose homeostasis Source: UniProtKB
Glucose import Source: UniProtKB
Insulin secretion Source: UniProtKB
Liver development Source: InterPro
Pancreas development Source: InterPro
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription initiation from RNA polymerase II promoter Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: BHF-UCL
Renal glucose absorption Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Hepatic adenomas familial (HEPAF):
Rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3).
Maturity-onset diabetes of the young 3 (MODY3):
A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Diabetes mellitus, insulin-dependent, 20 (IDDM20):
A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

Miyachi, Y., Miyazawa, T., & Ogawa, Y. (2022). HNF1A mutations and beta cell dysfunction in diabetes. International journal of molecular sciences, 23(6), 3222.

Li, L. M., Jiang, B. G., & Sun, L. L. (2022). HNF1A: from monogenic diabetes to type 2 diabetes and gestational diabetes mellitus. Frontiers in Endocrinology, 13, 829565.

Bonner, C., & Saponaro, C. (2022). Where to for precision treatment of HNF1A-MODY?. Diabetologia, 65(11), 1825-1829.

González, B. J., Zhao, H., Niu, J., Williams, D. J., Lee, J., Goulbourne, C. N., ... & Egli, D. (2022). Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiency. Communications Biology, 5(1), 779.

Kettunen, J. L., Rantala, E., Dwivedi, O. P., Isomaa, B., Sarelin, L., Kokko, P., ... & Tuomi, T. (2022). A multigenerational study on phenotypic consequences of the most common causal variant of HNF1A-MODY. Diabetologia, 1-12.

Cardenas-Diaz, F. L., Osorio-Quintero, C., Diaz-Miranda, M. A., Kishore, S., Leavens, K., Jobaliya, C., ... & Gadue, P. (2019). Modeling monogenic diabetes using human ESCs reveals developmental and metabolic deficiencies caused by mutations in HNF1A. Cell stem cell, 25(2), 273-289.

Valkovicova, T., Skopkova, M., Stanik, J., & Gasperikova, D. (2019). Novel insights into genetics and clinics of the HNF1A-MODY. Endocr Regul, 53(2), 110-134.

Locke, J. M., Saint-Martin, C., Laver, T. W., Patel, K. A., Wood, A. R., Sharp, S. A., ... & Weedon, M. N. (2018). The common HNF1A variant I27L is a modifier of age at diabetes diagnosis in individuals with HNF1A-MODY. Diabetes, 67(9), 1903-1907.

Abel, E. V., Goto, M., Magnuson, B., Abraham, S., Ramanathan, N., Hotaling, E., ... & Simeone, D. M. (2018). HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties. Elife, 7, e33947.

Liu, H. T., Liu, S., Liu, L., Ma, R. R., & Gao, P. (2018). EGR1-mediated transcription of lncRNA-HNF1A-AS1 promotes cell-cycle progression in gastric cancer. Cancer research, 78(20), 5877-5890.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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