Human AKAP4 ELISA Kit (V2LY-0626-LY4327)

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Tested Data
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Basic Information

Sensitivity
0.0076 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
94.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
A kinase (PRKA) anchor protein 4
Function
Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Biological Process
Establishment of protein localization Source: Ensembl
Flagellated sperm motility Source: UniProtKB
Motile cilium assembly Source: Ensembl
Protein localization Source: GO_Central
Signal transduction Source: ProtInc
Single fertilization Source: ProtInc
Transmembrane receptor protein serine/threonine kinase signaling pathway Source: Ensembl
Cellular Location
Flagellum. Localizes to the principle piece of the sperm flagellum.

Choowong‐in, P., Sattayasai, J., Poodendaen, C., & Iamsaard, S. (2021). Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS. Andrologia, 53(3), e13977.

Blommaert, D., Sergeant, N., Delehedde, M., Jouy, N., Mitchell, V., Franck, T., ... & Serteyn, D. (2019). Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology, 131, 52-60.

Nixon, B., Bernstein, I. R., Cafe, S. L., Delehedde, M., Sergeant, N., Anderson, A. L., ... & Bromfield, E. G. (2019). A kinase anchor protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in cell and developmental biology, 7, 319.

Li, Q., Tang, H., Hu, F., & Qin, C. (2018). Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells. Journal of cellular biochemistry, 119(12), 10013-10020.

Jumeau, F., Sigala, J., Dossou‐Gbete, F., Frimat, K., Barbotin, A. L., Buee, L., ... & Mitchell, V. (2018). A‐kinase anchor protein 4 precursor (pro‐AKAP4) in human spermatozoa. Andrology, 6(6), 854-859.

Kumar, V., Jagadish, N., & Suri, A. (2017). Role of A-Kinase anchor protein (AKAP4) in growth and survival of ovarian cancer cells. Oncotarget, 8(32), 53124.

Han, J., Gao, W., Su, D., & Liu, Y. (2017). Silencing of A-kinase anchor protein 4 (AKAP4) inhibits proliferation and progression of thyroid cancer. Oncology research, 25(6), 873.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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