Human HRH2 ELISA Kit (V2LY-0626-LY5686)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.015 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
40.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Histamine Receptor H2
Function
The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway (By similarity).
Biological Process
Chemical synaptic transmission Source: GO_Central
Gastric acid secretion Source: InterPro
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Immune response Source: ProtInc
Positive regulation of vasoconstriction Source: InterPro
Cellular Location
Cell membrane
Topology
Extracellular: 1-22
Helical: 23-44
Cytoplasmic: 45-57
Helical: 58-81
Extracellular: 82-92
Helical: 93-114
Cytoplasmic: 115-134
Helical: 135-159
Extracellular: 160-180
Helical: 181-204
Cytoplasmic: 205-234
Helical: 235-258
Extracellular: 259-267
Helical: 268-289
Cytoplasmic: 290-359

Ma, Q., Jiang, L., Chen, H., An, D., Ping, Y., Wang, Y., ... & Hu, W. (2023). Histamine H2 receptor deficit in glutamatergic neurons contributes to the pathogenesis of schizophrenia. Proceedings of the National Academy of Sciences, 120(9), e2207003120.

Patel, A., Marquez-Gomez, P. L., Torp, L. R., Gao, L., & Peralta-Yahya, P. (2023). Insight into the Mode of Action of 8-Hydroxyquinoline-Based Blockers on the Histamine Receptor 2. Biosensors, 13(6), 571.

Marquez-Gomez, P. L., Kruyer, N. S., Eisen, S. L., Torp, L. R., Howie, R. L., Jones, E. V., ... & Peralta-Yahya, P. (2022). Discovery of 8-hydroxyquinoline as a histamine receptor 2 blocker scaffold. ACS Synthetic Biology, 11(8), 2820-2828.

Fultz, R., Engevik, M. A., Shi, Z., Hall, A., Herrmann, B., Ganesh, B. P., ... & Versalovic, J. (2019). Phagocytosis by macrophages depends on histamine H2 receptor signaling and scavenger receptor 1. Microbiologyopen, 8(10), e908.

Shi, Z., Fultz, R. S., Engevik, M. A., Gao, C., Hall, A., Major, A., ... & Versalovic, J. (2019). Distinct roles of histamine H1-and H2-receptor signaling pathways in inflammation-associated colonic tumorigenesis. American Journal of Physiology-Gastrointestinal and Liver Physiology, 316(1), G205-G216.

Leary, P. J., Kronmal, R. A., Bluemke, D. A., Buttrick, P. M., Jones, K. L., Kao, D. P., ... & Bristow, M. R. (2018). Histamine H2 receptor polymorphisms, myocardial transcripts, and heart failure (from the multi-ethnic study of atherosclerosis and beta-blocker effect on remodeling and gene expression trial). The American journal of cardiology, 121(2), 256-261.

Singh, V., Gohil, N., & Ramírez‐García, R. (2018). New insight into the control of peptic ulcer by targeting the histamine H2 receptor. Journal of Cellular Biochemistry, 119(2), 2003-2011.

Moisejevs, G., Gailîte, L., Isajevs, S., Nikitina-Zaķe, L., Kempa, I., Jančiauskas, D., ... & Leja, M. (2018, December). Lack of Association Between Rs2067474 Polymorphism in the Histamine Receptor H2 Gene and Gastric Cancer In Latvian Population. In Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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