Human HSPA4 ELISA Kit (V2LY-0626-LY3591)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.018 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
94.3 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Heat Shock Protein Family A (Hsp70) Member 4
Biological Process
Chaperone-mediated protein complex assembly Source: BHF-UCL
Protein insertion into mitochondrial outer membrane Source: BHF-UCL
Response to unfolded protein Source: UniProtKB
Cellular Location
Cytoplasm

Yuan, X., Sun, X., Zhou, B., Zhao, S., Li, Y., & Ming, H. (2023). HSPA4 regulated glioma progression via activation of AKT signaling pathway. Biochemistry and Cell Biology, (ja).

Yang, Y., Zhang, S., Guan, J., Jiang, Y., Zhang, J., Luo, L., & Sun, C. (2022). SIRT1 attenuates neuroinflammation by deacetylating HSPA4 in a mouse model of Parkinson's disease. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1868(5), 166365.

Li, S. C., Lan, K. C., Hung, H. N., Huang, W. T., Lai, Y. J., Cheng, H. H., ... & Hsu, T. Y. (2022). HSPA4 is a biomarker of placenta accreta and enhances the angiogenesis ability of vessel endothelial cells. International Journal of Molecular Sciences, 23(10), 5682.

Sun, Q., Lv, Y., & Sun, W. (2022). Inhibition of DNAJC12 Inhibited Tumorigenesis of Rectal Cancer via Downregulating HSPA4 Expression. Evidence-based Complementary and Alternative Medicine: eCAM, 2022.

Shang, B. B., Chen, J., Wang, Z. G., & Liu, H. (2021). Significant correlation between HSPA4 and prognosis and immune regulation in hepatocellular carcinoma. PeerJ, 9, e12315.

Zhang, M., Dai, W., Li, Z., Tang, L., Chen, J., & Chen, C. (2021). HSPA4 knockdown retarded progression and development of colorectal cancer. Cancer Management and Research, 4679-4690.

Gu, Y., Liu, Y., Fu, L., Zhai, L., Zhu, J., Han, Y., ... & Cao, X. (2019). Tumor-educated B cells selectively promote breast cancer lymph node metastasis by HSPA4-targeting IgG. Nature medicine, 25(2), 312-322.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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