Human IFNL1 ELISA Kit (V2LY-0626-LY221)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0034 ng/mL
Detection Range
0.006-1.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
21.9 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Interferon Lambda 1
Function
Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
Biological Process
Defense response to virus Source: UniProtKB
Innate immune response Source: GO_Central
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of interleukin-13 production Source: UniProtKB
Negative regulation of interleukin-5 production Source: UniProtKB
Negative regulation of memory T cell differentiation Source: UniProtKB
Negative regulation of T cell differentiation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of type 2 immune response Source: UniProtKB
Positive regulation of immune response Source: UniProtKB
Positive regulation of interferon-gamma production Source: UniProtKB
Positive regulation of MHC class I biosynthetic process Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
Receptor signaling pathway via JAK-STAT Source: InterPro
Cellular Location
Secreted

Chua, K. J., Ling, H., Hwang, I. Y., Lee, H. L., March, J. C., Lee, Y. S., & Chang, M. W. (2022). An Engineered Probiotic Produces a Type III Interferon IFNL1 and Reduces Inflammations in in vitro Inflammatory Bowel Disease Models. ACS Biomaterials Science & Engineering.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IFNL1 protein (CAT#: V2LY-0526-LY4870)

Human Recombinant IFNL1 protein, His Tag (CAT#: V2LY-0526-LY4871)

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Human Recombinant IFNL1 protein (CAT#: V2LY-0526-LY4870)

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Isotype control

For research use only. Not intended for any clinical use.

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