Human ITIH5 ELISA Kit (V2LY-0626-LY3417)

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Tested Data
Request for COA
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Basic Information

Sensitivity
0.31 ng/mL
Detection Range
0.5-200 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
104.6 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
inter-alpha (globulin) inhibitor H5
Function
May act as a tumor suppressor.
Biological Process
Hyaluronan metabolic processIEA:InterPro
Cellular Location
Secreted

Rose, M., Huth, S., Wiesehöfer, M., Ehling, J., Henkel, C., Steitz, J., ... & Dahl, E. (2022). ITIH5-Derived Polypeptides Covering the VIT Domain Suppress the Growth of Human Cancer Cells In Vitro. Cancers, 14(3), 488.

Zou, L., He, H., Li, Z., Chen, O., Jia, X., & Zhang, H. (2021). Long noncoding RNA LINC00261 upregulates ITIH5 to impair tumorigenic ability of pancreatic cancer stem cells. Cell Death Discovery, 7(1), 220.

Young, E. D., Manley, S. J., Beadnell, T. C., Shearin, A. E., Sasaki, K., Zimmerman, R., ... & Welch, D. R. (2021). Suppression of pancreatic cancer liver metastasis by secretion-deficient ITIH5. British journal of cancer, 124(1), 166-175.

Liu, J., Cao, F., Li, X., Zhang, L., Liu, Z., Li, X., ... & Han, C. (2021). ITIH5, a p53-responsive gene, inhibits the growth and metastasis of melanoma cells by downregulating the transcriptional activity of KLF4. Cell Death & Disease, 12(5), 438.

Daum, A. K., Dittmann, J., Jansen, L., Peters, S., Dahmen, U., Heger, J. I., ... & Backsch, C. (2021). ITIH5 shows tumor suppressive properties in cervical cancer cells grown as multicellular tumor spheroids. American journal of translational research, 13(9), 10298.

Rose, M., Noetzel, E., Kistermann, J., Eschenbruch, J., Rushrush, S., Gan, L., ... & Dahl, E. (2021). The ECM modulator ITIH5 affects cell adhesion, motility and chemotherapeutic response of basal/squamous-like (BASQ) bladder cancer cells. Cells, 10(5), 1038.

Huth, S., Huth, L., Marquardt, Y., Fietkau, K., Dahl, E., Esser, P. R., ... & Baron, J. M. (2020). Inter-α-trypsin inhibitor heavy chain 5 (ITIH5) is a natural stabilizer of hyaluronan that modulates biological processes in the skin. Skin Pharmacology and Physiology, 33(4), 198-206.

Rose, M., Bringezu, S., Godfrey, L., Fiedler, D., Gaisa, N. T., Koch, M., ... & Dahl, E. (2020). ITIH5 and ECRG4 DNA methylation biomarker test (EI-BLA) for urine-based non-invasive detection of bladder cancer. International Journal of Molecular Sciences, 21(3), 1117.

Rose, M., Meurer, S. K., Kloten, V., Weiskirchen, R., Denecke, B., Antonopoulos, W., ... & Dahl, E. (2018). ITIH5 induces a shift in TGF‐β superfamily signaling involving Endoglin and reduces risk for breast cancer metastasis and tumor death. Molecular carcinogenesis, 57(2), 167-181.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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