Human ITPA ELISA Kit (V2LY-0626-LY2316)

Name: Human ITPA ELISA Kit
SKU: V2LY-0626-LY2316
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.05 ng/mL

Detection Range

0.1-30 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

21.4 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

inosine triphosphatase (nucleoside triphosphate pyrophosphatase)

Function

Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.

Biological Process

Chromosome organizationIEA:Ensembl
Deoxyribonucleoside triphosphate catabolic processIEA:UniProtKB-UniRule
ITP catabolic processIEA:Ensembl
Nucleoside triphosphate catabolic processManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm

Involvement in disease

Inosine triphosphate pyrophosphohydrolase deficiency (ITPAD):
A common inherited condition characterized by the abnormal accumulation of inosine triphosphate in erythrocytes. It might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs.
Developmental and epileptic encephalopathy 35 (DEE35):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE35 is characterized by onset of seizures in the first months of life associated with essentially no normal development. Many patients die in early childhood.

Straube, H., Straube, J., Rinne, J., Fischer, L., Niehaus, M., Witte, C. P., & Herde, M. (2023). An inosine triphosphate pyrophosphatase safeguards plant nucleic acids from aberrant purine nucleotides. New Phytologist, 237(5), 1759-1775.

Schroader, J. H., Handley, M. T., & Reddy, K. (2023). Inosine triphosphate pyrophosphatase: A guardian of the cellular nucleotide pool and potential mediator of RNA function. Wiley Interdisciplinary Reviews: RNA, e1790.

Zamzami, M. A. (2022). Inosine triphosphate pyrophosphatase (ITPase): Functions, mutations, polymorphisms and its impact on cancer therapies. Cells, 11(3), 384.

Vidal, A. E., Yagüe-Capilla, M., Martínez-Arribas, B., García-Caballero, D., Ruiz-Pérez, L. M., & González-Pacanowska, D. (2022). Inosine triphosphate pyrophosphatase from Trypanosoma brucei cleanses cytosolic pools from deaminated nucleotides. Scientific Reports, 12(1), 6408.

Scala, M., Wortmann, S. B., Kaya, N., Stellingwerff, M. D., Pistorio, A., Glamuzina, E., ... & Houlden, H. (2022). Clinico‐radiological features, molecular spectrum, and identification of prognostic factors in developmental and epileptic encephalopathy due to inosine triphosphate pyrophosphatase (ITPase) deficiency. Human mutation, 43(3), 403-419.

Zamzami, M. A. (2022). Inosine triphosphate pyrophosphatase (ITPase): Functions, mutations, polymorphisms and its impact on cancer therapies. Cells. 2022; 11: 384.

Houndonougbo, Y., Pugh, B., VanWormer, K., April, C., & Burgis, N. (2021). Structural dynamics of inosine triphosphate pyrophosphatase (ITPA) protein and two clinically relevant mutants: molecular dynamics simulations. Journal of Biomolecular Structure and Dynamics, 39(4), 1236-1247.

Peltenburg, N. C., Bierau, J., Bakker, J. A., Schippers, J. A., Lowe, S. H., Paulussen, A. D., ... & Verbon, A. (2018). Erythrocyte Inosine triphosphatase activity: A potential biomarker for adverse events during combination antiretroviral therapy for HIV. PLoS One, 13(1), e0191069.

Nyström, K., Wanrooij, P. H., Waldenström, J., Adamek, L., Brunet, S., Said, J., ... & Lagging, M. (2018). Inosine triphosphate pyrophosphatase dephosphorylates ribavirin triphosphate and reduced enzymatic activity potentiates mutagenesis in hepatitis C virus. Journal of virology, 92(19), 10-1128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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