Human KCNJ12 ELISA Kit (V2LY-0626-LY1962)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.021 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
49.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Potassium Voltage-Gated Channel Subfamily J Member 12
Function
Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Biological Process
Muscle contractionManual Assertion Based On ExperimentTAS:ProtInc
Potassium ion import across plasma membraneManual Assertion Based On ExperimentIBA:GO_Central
Potassium ion transportManual Assertion Based On ExperimentIDA:UniProtKB
Protein homotetramerizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of heart contractionManual Assertion Based On ExperimentTAS:ProtInc
Regulation of ion transmembrane transportManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cell membrane
Topology
Cytoplasmic: 1-82
Helical: 83-107
Extracellular: 108-123
Helical: 124-141
Pore-forming: 142-151
Extracellular: 152-156
Helical: 157-181
Cytoplasmic: 182-433

Wang, J., Wen, Y., Xu, J., Yue, B., Zhong, J., Zheng, L., ... & Huang, Y. (2022). Circ RIMKLB promotes myoblast proliferation and inhibits differentiation by sponging miR-29c to release KCNJ12. Epigenetics, 17(12), 1686-1700.

Chen, K., Hu, Y. Y., Wang, L. L., Xia, Y., Jiang, Q., Sun, L., ... & Li, D. S. (2022). Whole-Genome Sequencing Identified KCNJ12 and SLC25A5 Mutations in Port-Wine Stains. Frontiers in Medicine, 9, 905902.

Li, G., Guo, B. Y., Wang, H. D., Lin, G. T., Lan, T. J., Ying, H., & Xu, J. (2021). CircRNA hsa_circ_0014130 function as a miR-132-3p sponge for playing oncogenic roles in bladder cancer via upregulating KCNJ12 expression. Cell biology and toxicology, 1-18.

Zhang, X., Liao, C., Sun, K., Liu, L., & Xu, D. (2020, August). A Soluble Epoxide Hydrolase Inhibitor Upregulated KCNJ12 and KCNIP2 by Downregulating MicroRNA-29 in a Mouse Model of Myocardial Infarction. In The Heart Surgery Forum (Vol. 23, No. 5, pp. E579-E585).

Cheng, J., Peng, W., Cao, X., Huang, Y., Lan, X., Lei, C., & Chen, H. (2019). A Missense Mutation in KCNJ12 Was Strongly Associated with Beef Cattle Stature.

Cheng, J., Peng, W., Cao, X., Huang, Y., Lan, X., Lei, C., & Chen, H. (2019). Differential expression of KCNJ12 gene and association analysis of its missense mutation with growth traits in chinese cattle. Animals, 9(5), 273.

Zheng, L., Xu, J. W., Li, J. C., Wang, D. H., An, Q. M., Xu, L. N., ... & Huang, Y. Z. (2019). Distribution and association study in copy number variation of KCNJ12 gene across four Chinese cattle populations. Gene, 689, 90-96.

Khalilipour, N., Baranova, A., Jebelli, A., Heravi-Moussavi, A., Bruskin, S., & Abbaszadegan, M. R. (2018). Familial esophageal squamous cell carcinoma with damaging rare/germline mutations in KCNJ12/KCNJ18 and GPRIN2 genes. Cancer genetics, 221, 46-52.

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For research use only. Not intended for any clinical use.

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