Human KIRREL1 ELISA Kit (V2LY-0626-LY4785)

Name: Human KIRREL1 ELISA Kit
SKU: V2LY-0626-LY4785
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0098 ng/mL

Detection Range

0.02-6 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

83.5 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Kirre Like Nephrin Family Adhesion Molecule 1

Function

Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm (PubMed:31472902).
It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 (By similarity).

Biological Process

Cell-cell adhesionManual Assertion Based On ExperimentIBA:GO_Central
Cell-cell junction maintenanceManual Assertion Based On ExperimentIMP:UniProtKB
ExcretionIEA:Ensembl
Glomerular filtrationManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of protein phosphorylationIEA:Ensembl
Positive regulation of actin filament polymerizationIEA:Ensembl

Cellular Location

Cell membrane. Predominantly located at podocyte slit diaphragm.

Involvement in disease

Nephrotic syndrome 23 (NPHS23):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form that progresses to end-stage renal failure. NPHS23 is an autosomal recessive form characterized by onset of proteinuria in the first or second decade of life, and variable outcome. Some patients have normal renal function after many years, whereas others may progress to chronic kidney disease.

Topology

Extracellular: 17-499
Helical: 500-520
Cytoplasmic: 521-757

PTM

Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding (By similarity).
N-glycosylated.

Paul, A., Annunziato, S., Lu, B., Sun, T., Evrova, O., Planas-Paz, L., ... & Cong, F. (2022). Cell adhesion molecule KIRREL1 is a feedback regulator of Hippo signaling recruiting SAV1 to cell-cell contact sites. Nature Communications, 13(1), 930.

Gu, Y., Wang, Y., Sha, Z., He, C., Zhu, Y., Li, J., ... & Yu, F. X. (2022). Transmembrane protein KIRREL1 regulates Hippo signaling via a feedback loop and represents a therapeutic target in YAP/TAZ-active cancers. Cell Reports, 40(9).

Solanki, A. K., Widmeier, E., Arif, E., Sharma, S., Daga, A., Srivastava, P., ... & Nihalani, D. (2019). Mutations in KIRREL1, a slit diaphragm component, cause steroid-resistant nephrotic syndrome. Kidney international, 96(4), 883-889.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-KIRREL1 Recombinant Antibody (G-8) (CAT#: CBMAB-N0886-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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