Human LMOD1 ELISA Kit (V2LY-0626-LY829)

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Tested Data
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Basic Information

Sensitivity
0.041 ng/mL
Detection Range
0.1-38 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
67.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
leiomodin 1 (smooth muscle)
Function
Required for proper contractility of visceral smooth muscle cells (PubMed:28292896).
Mediates nucleation of actin filaments.
Biological Process
Actin filament organizationManual Assertion Based On ExperimentIBA:GO_Central
Actin nucleationManual Assertion Based On ExperimentIDA:UniProtKB
Muscle contractionManual Assertion Based On ExperimentIBA:GO_Central
Myofibril assemblyManual Assertion Based On ExperimentIBA:GO_Central
Pointed-end actin filament cappingIEA:InterPro
Positive regulation of actin filament polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Cytoplasm, myofibril, sarcomere
Cytoplasm, cytoskeleton
Colocalizes with actin filaments in sarcomeres.
Involvement in disease
Megacystis-microcolon-intestinal hypoperistalsis syndrome 3 (MMIHS3):
A form of megacystis-microcolon-intestinal hypoperistalsis syndrome, a congenital visceral myopathy primarily affecting females, and characterized by loss of smooth muscle contraction in the bladder and intestine. Affected individuals present at birth with functional obstruction of intestine, microcolon, dilation of bladder, and secondary hydronephrosis. The majority of cases have a fatal outcome due to malnutrition and sepsis, followed by multiorgan failure. MMIHS3 inheritance is autosomal recessive.

Fournier, N., & Fabre, A. (2022). Smooth muscle motility disorder phenotypes: A systematic review of cases associated with seven pathogenic genes (ACTG2, MYH11, FLNA, MYLK, RAD21, MYL9 and LMOD1). Intractable & Rare Diseases Research, 11(3), 113-119.

Tan, Y., Chen, Q., Pan, S., An, W., Xu, H., Xing, Y., & Zhang, J. (2022). LMOD1, an oncogene associated with Lauren classification, regulates the metastasis of gastric cancer cells through the FAK-AKT/mTOR pathway. BMC cancer, 22(1), 474.

Kosiński, K., Malinowski, D., Safranow, K., Dziedziejko, V., & Pawlik, A. (2022). PECAM1, COL4A2, PHACTR1, and LMOD1 Gene Polymorphisms in Patients with Unstable Angina. Journal of Clinical Medicine, 11(2), 373.

Liu, K., Lu, L., Chen, S., Gu, B., Cai, H., Wang, Y., & Cai, W. (2022). Loss‐of‐function variants within LMOD1 actin‐binding site 2 cause pediatric intestinal pseudo‐obstruction by impairing protein stability and actin nucleation. The FASEB Journal, 36(3), e22194.

Qiao, X., Fu, S., Duan, X., Zuo, Y., Zhu, X., Wang, H., & Wang, J. (2021). MYLK, CNN1, TAGLN and LMOD1 identified as potential prognostic biomarkers for bladder cancer using bioinformatics analysis.

Nanda, V., Wang, T., Pjanic, M., Liu, B., Quertermous, T., Miano, J., ... & Miller, C. L. (2018). Regulatory Mechanism of Lmod1 Mediated Predisposition to Coronary Artery Disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(Suppl_1), A619-A619.

Nanda, V., Wang, T., Pjanic, M., Liu, B., Nguyen, T., Matic, L. P., ... & Miller, C. L. (2018). Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus. PLoS genetics, 14(11), e1007755.

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For research use only. Not intended for any clinical use.

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