Human LPCAT1 ELISA Kit (V2LY-0626-LY5719)

Name: Human LPCAT1 ELISA Kit
SKU: V2LY-0626-LY5719
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.048 ng/mL

Detection Range

0.1-40 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

59.2 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

LPCAT1

Function

Exhibits acyltransferase activity (PubMed:21498505, PubMed:18156367).
Exhibits acetyltransferase activity (By similarity).
Activity is calcium-independent (By similarity).
Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed:21498505, PubMed:18156367).
Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity).
Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity).
Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (By similarity).
May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (By similarity).
Involved in the regulation of lipid droplet number and size (PubMed:25491198).

Biological Process

Negative regulation of phosphatidylcholine biosynthetic processIEA:Ensembl
Phosphatidic acid biosynthetic processTAS:Reactome
Phosphatidylcholine acyl-chain remodelingManual Assertion Based On ExperimentIDA:UniProtKB
Phosphatidylglycerol acyl-chain remodelingTAS:Reactome
Phospholipid biosynthetic processISS:UniProtKB
Positive regulation of protein catabolic processIEA:Ensembl
Retina development in camera-type eyeIEA:Ensembl
Surfactant homeostasisIEA:Ensembl

Cellular Location

Endoplasmic reticulum membrane
Golgi apparatus membrane
Cell membrane
Lipid droplet
May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm.

Topology

Cytoplasmic: 1-57
Helical: 58-78
Lumenal: 79-534

Huang, Y., Wang, Y., Wang, Y., Wang, N., Duan, Q., Wang, S., ... & Zheng, Y. (2022). LPCAT1 promotes cutaneous squamous cell carcinoma via EGFR-mediated protein kinase B/p38MAPK signaling pathways. Journal of Investigative Dermatology, 142(2), 303-313.

Ding, J., Ding, X., & Leng, Z. (2022). LPCAT1 promotes gefitinib resistance via upregulation of the EGFR/PI3K/AKT signaling pathway in lung adenocarcinoma. Journal of Cancer, 13(6), 1837.

He, R. Q., Li, J. D., Du, X. F., Dang, Y. W., Yang, L. J., Huang, Z. G., ... & Chen, G. (2021). LPCAT1 overexpression promotes the progression of hepatocellular carcinoma. Cancer cell international, 21(1), 1-17.

Tao, M., Luo, J., Gu, T., Yu, X., Song, Z., Jun, Y., ... & Wang, Q. (2021). LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma. Cell death & disease, 12(9), 845.

Wang, K., Wu, Z., Si, Y., Tang, W., Xu, X., Cheng, Y., & Lin, J. (2021). Identification of LPCAT1 expression as a potential prognostic biomarker guiding treatment choice in acute myeloid leukemia. Oncology Letters, 21(2), 1-1.

Ji, W., Peng, Z., Sun, B., Chen, L., Zhang, Q., Guo, M., & Su, C. (2021). LpCat1 promotes malignant transformation of hepatocellular carcinoma cells by directly suppressing STAT1. Frontiers in Oncology, 11, 678714.

Liu, Y., Yang, C., Zhang, Z., & Jiang, H. (2021). Gut microbiota dysbiosis accelerates prostate cancer progression through increased LPCAT1 expression and enhanced DNA repair pathways. Frontiers in oncology, 11, 679712.

Han, C., Yu, G., Mao, Y., Song, S., Li, L., Zhou, L., ... & Xu, B. (2020). LPCAT1 enhances castration resistant prostate cancer progression via increased mRNA synthesis and PAF production. PLoS One, 15(11), e0240801.

Wei, C., Dong, X., Lu, H., Tong, F., Chen, L., Zhang, R., ... & Dong, X. (2019). LPCAT1 promotes brain metastasis of lung adenocarcinoma by up-regulating PI3K/AKT/MYC pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-16.

Lebok, P., von Hassel, A., Meiners, J., Hube-Magg, C., Simon, R., Höflmayer, D., ... & Burandt, E. C. (2019). Up-regulation of lysophosphatidylcholine acyltransferase 1 (LPCAT1) is linked to poor prognosis in breast cancer. Aging (Albany NY), 11(18), 7796.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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