Human MAP4 ELISA Kit (V2LY-0626-LY3100)

Name: Human MAP4 ELISA Kit
SKU: V2LY-0626-LY3100
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.009 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

121.0 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

microtubule-associated protein 4

Function

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

Biological Process

Cell divisionManual Assertion Based On ExperimentIMP:MGI
Establishment of spindle orientationManual Assertion Based On ExperimentIMP:MGI
Microtubule cytoskeleton organizationISS:ARUK-UCL
Microtubule slidingManual Assertion Based On ExperimentIMP:MGI
Mitotic spindle organizationManual Assertion Based On ExperimentIMP:MGI
Negative regulation of non-motile cilium assemblyManual Assertion Based On ExperimentIMP:GO_Central
Neuron projection developmentManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center
Recruitment to microtubule is inhibited by microtubules polyglutamylation.

PTM

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity).
Phosphorylation on Ser-787 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 3 is phosphorylated on Ser-337 and Ser-338.

Yu, X., Chen, X., Amrute-Nayak, M., Allgeyer, E., Zhao, A., Chenoweth, H., ... & Li, X. (2021). MARK4 controls ischaemic heart failure through microtubule detyrosination. Nature, 594(7864), 560-565.

Thapa, N., Chen, M., Horn, H. T., Choi, S., Wen, T., & Anderson, R. A. (2020). Phosphatidylinositol 3-kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4. Nature cell biology, 22(11), 1357-1370.

Li, L., Zhang, Q., Lei, X., Huang, Y., & Hu, J. (2020). MAP4 as a new candidate in cardiovascular disease. Frontiers in Physiology, 11, 1044.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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