Human MYOF ELISA Kit (V2LY-0626-LY4918)

Name: Human MYOF ELISA Kit
SKU: V2LY-0626-LY4918
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.023 ng/mL

Detection Range

0.05-10 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

234.7 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

MYOFERLIN

Function

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).

Biological Process

Blood circulation Source: ProtInc
Membrane fusion Source: GO_Central
Muscle contraction Source: ProtInc
Myoblast fusion Source: InterPro
Plasma membrane organization Source: GO_Central
Plasma membrane repair Source: UniProtKB
T-tubule organization Source: GO_Central

Cellular Location

Nucleus
Nucleus membrane
Plasma membrane
Cell membrane
Other locations
Cytoplasmic vesicle membrane
Note: Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.

Involvement in disease

Angioedema, hereditary, 7 (HAE7):
A form of angioedema, a disorder characterized by episodic local swelling involving subcutaneous or submucous tissue of the upper respiratory and gastrointestinal tracts, face, extremities, and genitalia. HAE7 is an autosomal dominant form characterized by onset of recurrent swelling of the face, lips, and oral mucosa in the second decade.

Topology

Cytoplasmic: 1-2025
Helical: 2026-2046
Extracellular: 2047-2061

Zhang, Y., Liu, W., Yuan, W., Cai, Z., Ye, G., Zheng, G., ... & Wang, P. (2022). Impairment of APPL1/Myoferlin facilitates adipogenic differentiation of mesenchymal stem cells by blocking autophagy flux in osteoporosis. Cellular and Molecular Life Sciences, 79(9), 488.

Shi, H., Cheng, Y., Shi, Q., Liu, W., Yang, X., Wang, S., ... & Fang, H. (2022). Myoferlin disturbs redox equilibrium to accelerate gastric cancer migration. Frontiers in Oncology, 12, 905230.

Barefield, D. Y., Sell, J. J., Tahtah, I., Kearns, S. D., McNally, E. M., & Demonbreun, A. R. (2021). Loss of dysferlin or myoferlin results in differential defects in excitation–contraction coupling in mouse skeletal muscle. Scientific reports, 11(1), 15865.

Pi, R., Chen, Y., Du, Y., & Dong, S. (2021). Comprehensive analysis of myoferlin in human pancreatic cancer via bioinformatics. BioMed Research International, 2021.

Gupta, S., Yano, J., Mercier, V., Htwe, H. H., Shin, H. R., Rademaker, G., ... & Perera, R. M. (2021). Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth. Nature cell biology, 23(3), 232-242.

Nozato, Y., Takami, Y., Yamamoto, K., Nagasawa, M., Nozato, S., Imaizumi, Y., ... & Rakugi, H. (2020). Novel properties of myoferlin in glucose metabolism via pathways involving modulation of adipose functions. The FASEB Journal, 34(2), 2792-2811.

Gu, H., Peng, Y., & Chen, Y. (2020). An emerging therapeutic approach by targeting myoferlin (MYOF) for malignant tumors. Current Topics in Medicinal Chemistry, 20(17), 1509-1515.

Cox, A., Zhao, C., Tolkach, Y., Nettersheim, D., Schmidt, D., Kristiansen, G., ... & Ellinger, J. (2020). Knockdown of myoferlin suppresses migration and invasion in clear-cell renal-cell carcinoma. Anticancer Research, 40(6), 3119-3128.

Han, S., Cui, C., He, H., Shen, X., Chen, Y., Wang, Y., ... & Yin, H. (2019). Myoferlin regulates Wnt/β-catenin signaling-mediated skeletal muscle development by stabilizing dishevelled-2 against autophagy. International Journal of Molecular Sciences, 20(20), 5130.

Dong, Y., Kang, H., Liu, H., Wang, J., Guo, Q., Song, C., ... & Li, F. (2019). Myoferlin, a membrane protein with emerging oncogenic roles. BioMed Research International, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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