Human NAP1L4 ELISA Kit (V2LY-0626-LY4979)

Name: Human NAP1L4 ELISA Kit
SKU: V2LY-0626-LY4979
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0091 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

42.8 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Nucleosome Assembly Protein 1 Like 4

Function

Acts as histone chaperone in nucleosome assembly.

Biological Process

Nucleosome assembly Source: UniProtKB

Cellular Location

Nucleus
Cytoplasm 2 Publications
Note: Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593).

PTM

Phosphorylated at the G0/G1 boundary but it is not phosphorylated in S-phase. Phosphorylated protein remains in the cytoplasm in a complex with histones during the G0/G1 transition, whereas dephosphorylation triggers its transport into the nucleus at the G1/S-boundary.
Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human (By similarity).

Dominguez, F., Shiliaev, N., Lukash, T., Agback, P., Palchevska, O., Gould, J. R., ... & Frolov, I. (2021). NAP1L1 and NAP1L4 binding to hypervariable domain of chikungunya virus nsP3 protein is bivalent and requires phosphorylation. Journal of Virology, 95(16), 10-1128.

Wang, S., Ren, X., Li, J., Lin, C., Zhou, J., Zhou, J., & Gu, J. (2020). NAP1L4 inhibits porcine circovirus type 2 replication via IFN-β signaling pathway. Veterinary Microbiology, 246, 108692.

Cheng, Z., Luo, Y., Zhang, Y., Wang, Y., Chen, Y., Xu, Y., ... & Zhang, G. (2020). A novel NAP1L4/NUTM1 fusion arising from translocation t (11; 15)(p15; q12) in a myeloid neoplasm with eosinophilia and rearrangement of PDGFRA highlights an unusual clinical feature and therapeutic reaction. Annals of Hematology, 99, 1561-1564.

Tanaka, T., Hozumi, Y., Martelli, A. M., Iino, M., & Goto, K. (2019). Nucleosome assembly proteins NAP1L1 and NAP1L4 modulate p53 acetylation to regulate cell fate. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(12), 118560.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-NAP1L4 Recombinant Antibody (CBWJN-0644) (CAT#: CBMAB-N1148-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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