Human NEU1 ELISA Kit (V2LY-0626-LY1163)

Name: Human NEU1 ELISA Kit
SKU: V2LY-0626-LY1163
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.045 ng/mL

Detection Range

0.1-40 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

45.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Neuraminidase 1

Function

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.

Biological Process

Ganglioside catabolic process Source: GO_Central
Oligosaccharide catabolic process Source: UniProtKB

Cellular Location

Lysosome
Lysosome membrane
Lysosome lumen
Plasma membrane
Cell membrane
Other locations
Cytoplasmic vesicle
Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.

Involvement in disease

Sialidosis (SIALIDOSIS):
Lysosomal storage disease occurring as two types with various manifestations. Type 1 sialidosis (cherry red spot-myoclonus syndrome or normosomatic type) is late-onset and it is characterized by the formation of cherry red macular spots in childhood, progressive debilitating myoclonus, insiduous visual loss and rarely ataxia. The diagnosis can be confirmed by the screening of the urine for sialyloligosaccharides. Type 2 sialidosis (also known as dysmorphic type) occurs as several variants of increasing severity with earlier age of onset. It is characterized by the presence of abnormal somatic features including coarse facies and dysostosis multiplex, vertebral deformities, mental retardation, cherry-red spot/myoclonus, sialuria, cytoplasmic vacuolation of peripheral lymphocytes, bone marrow cells and conjunctival epithelial cells.

PTM

N-glycosylated.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.

Chen, Q. Q., Liu, K., Shi, N., Ma, G., Wang, P., Xie, H. M., ... & Zhang, L. (2023). Neuraminidase 1 promotes renal fibrosis development in male mice. Nature Communications, 14(1), 1713.

Toussaint, K., Appert-Collin, A., Morjani, H., Albrecht, C., Sartelet, H., Romier-Crouzet, B., ... & Bennasroune, A. (2022). Neuraminidase-1: a sialidase involved in the development of cancers and metabolic diseases. Cancers, 14(19), 4868.

Wang, Q., Chen, Z., Peng, X., Zheng, Z., Le, A., Guo, J., ... & Zhu, J. (2021). Neuraminidase 1 exacerbating aortic dissection by governing a pro-inflammatory program in macrophages. Frontiers in Cardiovascular Medicine, 8, 788645.

Chen, Q. Q., Ma, G., Liu, J. F., Cai, Y. Y., Zhang, J. Y., Wei, T. T., ... & Qi, L. W. (2021). Neuraminidase 1 is a driver of experimental cardiac hypertrophy. European Heart Journal, 42(36), 3770-3782.

Heimerl, M., Sieve, I., Ricke-Hoch, M., Erschow, S., Battmer, K., Scherr, M., & Hilfiker-Kleiner, D. (2020). Neuraminidase-1 promotes heart failure after ischemia/reperfusion injury by affecting cardiomyocytes and invading monocytes/macrophages. Basic Research in Cardiology, 115, 1-17.

Allendorf, D. H., Franssen, E. H., & Brown, G. C. (2020). Lipopolysaccharide activates microglia via neuraminidase 1 desialylation of toll‐like receptor 4. Journal of Neurochemistry, 155(4), 403-416.

Kong, F., Li, N., Tu, T., Tao, Y., Bi, Y., Yuan, D., ... & Tang, R. (2020). Hepatitis B virus core protein promotes the expression of neuraminidase 1 to facilitate hepatocarcinogenesis. Laboratory Investigation, 100(12), 1602-1617.

Albrecht, C., Kuznetsov, A. S., Appert-Collin, A., Dhaideh, Z., Callewaert, M., Bershatsky, Y. V., ... & Bennasroune, A. (2020). Transmembrane peptides as a new strategy to inhibit neuraminidase-1 activation. Frontiers in Cell and Developmental Biology, 8, 611121.

Thulasiraman, P., Kerr, K., McAlister, K., Hardisty, S., Wistner, A., & McCullough, I. (2019). Neuraminidase 1 regulates proliferation, apoptosis and the expression of Cadherins in mammary carcinoma cells. Molecular and Cellular Biochemistry, 462, 207-215.

Bennasroune, A., Romier-Crouzet, B., Blaise, S., Laffargue, M., Efremov, R. G., Martiny, L., ... & Duca, L. (2019). Elastic fibers and elastin receptor complex: Neuraminidase-1 takes the center stage. Matrix Biology, 84, 57-67.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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